Intracellular release of 17-β estradiol from cationic polyamidoamine dendrimer surface-modified poly (lactic-co-glycolic acid) microparticles improves osteogenic differentiation of human mesenchymal stromal cells

Liu Hong; Yogita Krishnamachari; Denise Seabold; Vijaya Joshi; Galen Schneider; Aliasger K Salem

doi:10.1089/ten.tec.2010.0388

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Intracellular release of 17-β estradiol from cationic polyamidoamine dendrimer surface-modified poly (lactic-co-glycolic acid) microparticles improves osteogenic differentiation of human mesenchymal stromal cells

Journal article

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Peer reviewed

Intracellular release of 17-β estradiol from cationic polyamidoamine dendrimer surface-modified poly (lactic-co-glycolic acid) microparticles improves osteogenic differentiation of human mesenchymal stromal cells

Liu Hong, Yogita Krishnamachari, Denise Seabold, Vijaya Joshi, Galen Schneider and Aliasger K Salem

Tissue engineering. Part C, Methods, Vol.17(3), pp.319-325

03/2011

DOI: 10.1089/ten.tec.2010.0388

PMCID: PMC3045071

PMID: 20883116

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Abstract

Human bone marrow mesenchymal stromal cells (MSCs) are considered a potential cell source for MSC-based bone regeneration, but improvements in the proliferation and differentiation capacity of MSCs are necessary for practical applications. Estrogen effectively improves MSC capabilities and has strong potential as a regulator of MSCs. The aim of this study was to develop a delivery system that provides intracellular release of estrogen and test its ability to improve osteogenic differentiation of MSCs. Biodegradable poly (lactic-co-glycolic acid) (PLGA) microparticles were developed that entrap 17-β estradiol (E2) and provide intracellular release of E2. The results show that we can prepare PLGA particles with efficient loading of E2 and maintain release of E2 up to 7 days. Surface modifying E2-loaded PLGA particles with cationic polyamidoamine dendrimers enabled increased uptake by human MSCs. Human MSC uptake of the E2-loaded PLGA particles significantly upregulates osteogenic differentiation markers of alkaline phosphatase and osteocalcin. In conclusion, cationic-modified PLGA particles can serve as a tool for intracellular delivery of estrogen to effectively execute estrogen regulation of MSCs. This approach has the potential to improve the osteogenic capabilities of MSCs and to develop appropriate environments of implantation for MSC-based bone tissue engineering.

Biomarkers - metabolism

Estradiol - metabolism

Mesenchymal Stromal Cells - drug effects

Solvents

Receptors, Estrogen - metabolism

Humans

Osteogenesis - drug effects

Stromal Cells - metabolism

Mesenchymal Stromal Cells - metabolism

Polyglycolic Acid - pharmacology

Microscopy, Confocal

Microspheres

Mesenchymal Stromal Cells - cytology

Cell Differentiation - drug effects

Intracellular Space - metabolism

Stromal Cells - drug effects

Surface Properties - drug effects

Cations

Dendrimers - pharmacology

Lactic Acid - pharmacology

Stromal Cells - cytology

Details

Title: Subtitle: Intracellular release of 17-β estradiol from cationic polyamidoamine dendrimer surface-modified poly (lactic-co-glycolic acid) microparticles improves osteogenic differentiation of human mesenchymal stromal cells
Creators: Liu Hong - Dows Institute for Dental Research, Collage of Dentistry, University of Iowa, Iowa City, Iowa, USA. liu-hong@uiowa.edu
Yogita Krishnamachari
Denise Seabold
Vijaya Joshi
Galen Schneider
Aliasger K Salem
Resource Type: Journal article
Publication Details: Tissue engineering. Part C, Methods, Vol.17(3), pp.319-325
DOI: 10.1089/ten.tec.2010.0388
PMID: 20883116
PMCID: PMC3045071
NLM abbreviation: Tissue Eng Part C Methods
ISSN: 1937-3392
eISSN: 1937-3392
Publisher: United States
Grant note: 1R21CA13345-01 / NCI NIH HHS 1R21CA128414-01A2 / NCI NIH HHS
Language: English
Date published: 03/2011
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Dentistry Administration; Prosthodontics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering
Record Identifier: 9983985715302771

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