Journal article
Intracellular staining for TNF and IFN-γ detects different frequencies of antigen-specific CD8 + T cells
Journal of immunological methods, Vol.238(1), pp.107-117
2000
DOI: 10.1016/S0022-1759(00)00153-8
PMID: 10758241
Abstract
CD8
+ T lymphocytes are important mediators of adaptive immunity against certain viral, protozoan and bacterial pathogens. Activated CD8
+ T cells are able to induce cytolysis of infected cells (perforin and CD95–CD95L mediated pathways) and also elaborate cytokines, including IFN-γ and TNF after appropriate MHC class I-peptide recognition. New technologies for the detection of antigen-specific CD8
+ T cells, including tetrameric MHC class I-peptide complexes, intracellular IFN-γ staining and IFN-γ ELISPOT analysis have revised our understanding of the magnitude of the CD8
+ T cell response to infection. Here, using intracellular cytokine staining, we compare detection of IFN-γ and TNF in the analysis of pathogen-specific CD8
+ T cell lines and CD8
+ T cells after primary viral infection (LCMV) or secondary bacterial infection (
Listeria monocytogenes). Under multiple conditions and with multiple epitopes, we find that staining for intracellular IFN-γ consistently detects a higher frequency of antigen-specific CD8
+ T cells than detection of intracellular TNF. However, (a) intracellular staining for TNF can be used to detect antigen-specific CD8
+ T cell responses and (b) intracellular staining for cytokines is a useful approach for in vitro characterization of antigen-specific CD8
+ T cell lines.
Details
- Title: Subtitle
- Intracellular staining for TNF and IFN-γ detects different frequencies of antigen-specific CD8 + T cells
- Creators
- Vladimir P BadovinacJohn T Harty
- Resource Type
- Journal article
- Publication Details
- Journal of immunological methods, Vol.238(1), pp.107-117
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S0022-1759(00)00153-8
- PMID
- 10758241
- ISSN
- 0022-1759
- eISSN
- 1872-7905
- Language
- English
- Date published
- 2000
- Academic Unit
- Microbiology and Immunology; Pathology
- Record Identifier
- 9984046927402771
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