Journal article
Intracerebroventricular calcitonin prevents stress-induced gastric dysfunction
The Journal of surgical research, Vol.110(1), pp.188-192
2003
DOI: 10.1016/S0022-4804(03)00031-3
PMID: 12697399
Abstract
Background. Restraint stress produces gastric hypercontractility and acidity leading to stress ulceration. Intracerebroventricular (ICV) salmon calcitonin (sCT) decreases restraint injury and acidity, but its effects on restraint-induced hypercontractility are unknown.
Methods. Using stereotactic guidance, ICV catheters were placed into the lateral ventricle of adult male rats and calibrated gastric strain gauge transducers were implanted 5 days prior to restraint stress. sCT rats (
n = 8) were pretreated with 5 μg of calcitonin ICV (10 μl volume), while controls (
n = 10) received 10 μl of ICV saline prior to restraint for 2 h at 20°C followed by 2 h at 4°C. Gastric motility data were collected with AT-CODAS and analyzed with ADVANCED CODAS. Gastric volume, pH, and lesions were recorded following the stress.
Results. ICV calcitonin prevented gastric mucosal injury in all animals (0% vs 100%,
P < .01) and elevated pH slightly (2.5 ± .3 vs 1.6 ± .1,
P < .05). Stress caused the force of contractions to increase from 0.35 ± .1 to 1.38 ± .4 g in controls (
P < .01), while treated animal’s force fell from .42 ± .1 to 0.2 ± .05 g (
P < .01 vs control). Stress did not affect contractions/min (3.4 + .6 vs 3.5 + .3), but sCT increased frequency (2.5 + .4 to 5.0 + .2,
P < .01). Stress prolonged contraction duration (11.5 + 1 to 16.5 + 1.7 s,
P < .01), but stress’s effect was prevented by sCT (11.0 + .5 to 11.2 + .3,
P < .01 vs control).
Conclusions. Pretreatment with 5 μg central sCT prevents the increased amplitude and duration of gastric contractions produced by restraint stress for 2 h, in association with gastroprotection.
Details
- Title: Subtitle
- Intracerebroventricular calcitonin prevents stress-induced gastric dysfunction
- Creators
- Derek DuBay - University of IowaKimberly S Ephgrave - University of IowaJoseph J Cullen - University of IowaKimberly A Broadhurst - University of Iowa
- Resource Type
- Journal article
- Publication Details
- The Journal of surgical research, Vol.110(1), pp.188-192
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S0022-4804(03)00031-3
- PMID
- 12697399
- ISSN
- 0022-4804
- eISSN
- 1095-8673
- Language
- English
- Date published
- 2003
- Academic Unit
- Surgery; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984313069202771
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