Intracochlear Electrical Stimulation Suppresses Apoptotic Signaling in Rat Spiral Ganglion Neurons after Deafening in Vivo

Jonathan C Kopelovich; Alain P Cagaanan; Charles A Miller; Paul J Abbas; Steven H Green

doi:10.1177/0194599813498702

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Intracochlear Electrical Stimulation Suppresses Apoptotic Signaling in Rat Spiral Ganglion Neurons after Deafening in Vivo

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Intracochlear Electrical Stimulation Suppresses Apoptotic Signaling in Rat Spiral Ganglion Neurons after Deafening in Vivo

Jonathan C Kopelovich, Alain P Cagaanan, Charles A Miller, Paul J Abbas and Steven H Green

Otolaryngology-head and neck surgery, Vol.149(5), pp.745-752

11/2013

DOI: 10.1177/0194599813498702

PMCID: PMC4043189

PMID: 23907267

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https://www.ncbi.nlm.nih.gov/pmc/articles/4043189View

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Abstract

Objective To establish the intracellular consequences of electrical stimulation to spiral ganglion neurons after deafferentation. Here we use a rat model to determine the effect of both low and high pulse rate acute electrical stimulation on activation of the proapoptotic transcription factor Jun in deafferented spiral ganglion neurons in vivo. Study Design Experimental animal study. Setting Hearing research laboratories of the University of Iowa Departments of Biology and Otolaryngology. Methods A single electrode was implanted through the round window of kanamycin-deafened rats at either postnatal day 32 (P32, n = 24) or P60 (n = 22) for 4 hours of stimulation (monopolar, biphasic pulses, amplitude twice electrically evoked auditory brainstem response [eABR] threshold) at either 100 or 5000 Hz. Jun phosphorylation was assayed by immunofluorescence to quantitatively assess the effect of electrical stimulation on proapoptotic signaling. Results Jun phosphorylation was reliably suppressed by 100 Hz stimuli in deafened cochleae of P32 but not P60 rats. This effect was not significant in the basal cochlear turns. Stimulation frequency may be consequential: 100 Hz was significantly more effective than was 5 kHz stimulation in suppressing phospho-Jun. Conclusions Suppression of Jun phosphorylation occurs in deafferented spiral ganglion neurons after only 4 hours of electrical stimulation. This finding is consistent with the hypothesis that electrical stimulation can decrease spiral ganglion neuron death after deafferentation.

Details

Title: Subtitle: Intracochlear Electrical Stimulation Suppresses Apoptotic Signaling in Rat Spiral Ganglion Neurons after Deafening in Vivo
Creators: Jonathan C Kopelovich - Department of Otolaryngology Head and Neck Surgery, University of Iowa, Iowa City, Iowa, USA
Alain P Cagaanan - Department of Biology, University of Iowa, Iowa City, Iowa, USA
Charles A Miller - Department of Communication Sciences and Disorders, University of Iowa, Iowa City, Iowa, USA
Paul J Abbas - Department of Otolaryngology Head and Neck Surgery, University of Iowa, Iowa City, Iowa, USA, Department of Communication Sciences and Disorders, University of Iowa, Iowa City, Iowa, USA
Steven H Green - Department of Otolaryngology Head and Neck Surgery, University of Iowa, Iowa City, Iowa, USA, Department of Biology, University of Iowa, Iowa City, Iowa, USA
Resource Type: Journal article
Publication Details: Otolaryngology-head and neck surgery, Vol.149(5), pp.745-752
DOI: 10.1177/0194599813498702
PMID: 23907267
PMCID: PMC4043189
NLM abbreviation: Otolaryngol Head Neck Surg
ISSN: 0194-5998
eISSN: 1097-6817
Grant note: DOI: 10.13039/100000002, name: National Institutes of Health, award: R01 DC002961; DOI: 10.13039/100000002, name: National Institutes of Health, award: P30 DC010362; DOI: 10.13039/100005193, name: American Academy of Otolaryngology-Head and Neck Surgery; DOI: 10.13039/100000002, name: National Institutes of Health, award: T32 DC000040
Language: English
Date published: 11/2013
Academic Unit: Communication Sciences and Disorders; Biology; Otolaryngology
Record Identifier: 9984217414902771

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