Journal article
Intradermal vaccination with hollow microneedles: A comparative study of various protein antigen and adjuvant encapsulated nanoparticles
Journal of controlled release, Vol.266, pp.109-118
11/28/2017
DOI: 10.1016/j.jconrel.2017.09.021
PMID: 28943194
Abstract
In this study, we investigated the potential of intradermal delivery of nanoparticulate vaccines to modulate the immune response of protein antigen using hollow microneedles. Four types of nanoparticles covering a broad range of physiochemical parameters, namely poly (lactic-co-glycolic) (PLGA) nanoparticles, liposomes, mesoporous silica nanoparticles (MSNs) and gelatin nanoparticles (GNPs) were compared. The developed nanoparticles were loaded with a model antigen (ovalbumin (OVA)) with and without an adjuvant (poly(I:C)), followed by the characterization of size, zeta potential, morphology, and loading and release of antigen and adjuvant. An in-house developed hollow-microneedle applicator was used to inject nanoparticle suspensions precisely into murine skin at a depth of about 120 mu m. OVA/poly(I:C)-loaded nanoparticles and OVA/poly(I:C) solution elicited similarly strong total IgG and IgG1 responses. However, the co-encapsulation of OVA and poly (I:C) in nanoparticles significantly increased the IgG2a response compared to OVA/poly(I:C) solution. PLGA nanoparticles and liposomes induced stronger IgG2a responses than MSNs and GNPs, correlating with sustained release of the antigen and adjuvant and a smaller nanoparticle size. When examining cellular responses, the highest CD8(+) and CD4(+) T cell responses were induced by OVA/poly(I:C)-loaded liposomes. In conclusion, the applicator controlled hollow microneedle delivery is an excellent method for intradermal injection of nanoparticle vaccines, allowing selection of optimal nanoparticle formulations for humoral and cellular immune responses.
Details
- Title: Subtitle
- Intradermal vaccination with hollow microneedles: A comparative study of various protein antigen and adjuvant encapsulated nanoparticles
- Creators
- Guangsheng Du - Leiden UniversityRania M. Hathout - Centre for Human Drug ResearchMaha Nasr - Centre for Human Drug ResearchM. Reza Nejadnik - Centre for Human Drug ResearchJing Tu - Leiden UniversityRoman I. Koning - Leiden University Medical CenterAbraham J. Koster - Leiden University Medical CenterBram Slutter - Leiden UniversityAlexander Kros - Leiden UniversityWim Jiskoot - Leiden UniversityJoke A. Bouwstra - Centre for Human Drug ResearchJuha Monkare - Leiden University
- Resource Type
- Journal article
- Publication Details
- Journal of controlled release, Vol.266, pp.109-118
- DOI
- 10.1016/j.jconrel.2017.09.021
- PMID
- 28943194
- NLM abbreviation
- J Control Release
- ISSN
- 0168-3659
- eISSN
- 1873-4995
- Publisher
- Elsevier
- Number of pages
- 10
- Grant note
- Chinese Scholarship Council; China Scholarship Council 115363 / Innovative Medicines Initiative Joint Undertaking European Union's Seventh Framework Programme (FP7)
- Language
- English
- Date published
- 11/28/2017
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics
- Record Identifier
- 9984420836502771
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