Intragenic and extragenic suppression of a mutation in herpes simplex virus 1 UL34 that affects both nuclear envelope targeting and membrane budding

Richard J Roller; Alison C Haugo; Nora J Kopping

doi:10.1128/JVI.05730-11

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Intragenic and extragenic suppression of a mutation in herpes simplex virus 1 UL34 that affects both nuclear envelope targeting and membrane budding

Journal article

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Peer reviewed

Intragenic and extragenic suppression of a mutation in herpes simplex virus 1 UL34 that affects both nuclear envelope targeting and membrane budding

Richard J Roller, Alison C Haugo and Nora J Kopping

Journal of virology, Vol.85(22), pp.11615-11625

11/2011

DOI: 10.1128/JVI.05730-11

PMCID: PMC3209301

PMID: 21900173

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Abstract

Late in infection herpesviruses move DNA-filled capsids from the nucleus to the cytoplasm by enveloping DNA-containing capsids at the inner nuclear membrane (INM) and deenveloping them at the outer nuclear membrane. This process requires two conserved herpesvirus proteins, pUL31 and pUL34. Interaction between pUL34 and pUL31 is essential for targeting both proteins to the nuclear envelope (NE), and sequences that mediate the targeting interaction have been mapped in both proteins. Here, we show that a mutation in the INM-targeting domain of pUL34 fails to support production of infectious virus or plaque formation. The mutation results in multiple defects, including impaired interaction between pUL34 and pUL31, poor NE targeting of pUL34, and misregulated, capsid-independent budding of the NE. The mutant defects in virus production, plaque formation, and pUL31 interaction can be suppressed by other mutations in the INM-targeting domain of pUL31 and by additional mutations in the pUL34 coding sequence.

Virus Release

Cercopithecus aethiops

Viral Plaque Assay

Viral Proteins - genetics

Nuclear Envelope - virology

Viral Proteins - metabolism

Protein Interaction Mapping

Nuclear Envelope - metabolism

Animals

Protein Binding

Herpesvirus 1, Human - genetics

Cell Membrane - metabolism

Protein Interaction Domains and Motifs

Nuclear Proteins - genetics

Vero Cells

Herpesvirus 1, Human - pathogenicity

Suppression, Genetic

Details

Title: Subtitle: Intragenic and extragenic suppression of a mutation in herpes simplex virus 1 UL34 that affects both nuclear envelope targeting and membrane budding
Creators: Richard J Roller - Department of Microbiology, University of Iowa, BSB 3-432, Iowa City, IA 52242, USA. richard-roller@uiowa.edu
Alison C Haugo
Nora J Kopping
Resource Type: Journal article
Publication Details: Journal of virology, Vol.85(22), pp.11615-11625
Publisher: United States
DOI: 10.1128/JVI.05730-11
PMID: 21900173
PMCID: PMC3209301
ISSN: 0022-538X
eISSN: 1098-5514
Grant note: AI 41478 / NIAID NIH HHS R01 AI041478 / NIAID NIH HHS
Language: English
Date published: 11/2011
Academic Unit: Microbiology and Immunology
Record Identifier: 9984001162102771

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