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Intragenic mutations of the p16 super(INK4), p15 super(INK4B) and p18 genes in primary non-small-cell lung cancers
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Intragenic mutations of the p16 super(INK4), p15 super(INK4B) and p18 genes in primary non-small-cell lung cancers

M R Rusin, A Okamoto, M Chorazy, K Czyzewski, J Harasim, E A Spillare, K Hagiwara, S P Hussain, Yue Xiong, D J Demetrick, …
International journal of cancer, Vol.65(6), pp.734-739
03/01/1996
DOI: 10.1002/(SICI)1097-0215(19960315)65:6<734::AID-IJC4>3.0.CO;2-%23

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Abstract

The p15 super(INK4B), p16 super(INK4) and p18 genes are members of the gene family coding for inhibitors of cyclin-dependent kinases 4 and 6. p15 super(INK4B) and p 16 super(INK4) are located at 9p21, a chromosomal region frequently deleted in many human neoplasms. To examine the role of these 3 genes in lung carcinogenesis, somatic mutations within the genes were analyzed by single-strand conformation polymorphism and DNA sequencing in 71 non-small-cell lung cancer (NSCLC) samples. Six somatic mutations in the p16 super(INK4) gene and 3 cases with a polymorphic allele were observed. Loss of heterozygosity in the p18 gene was found in 1 sample. We did not find any intragenic mutations in the p15 super(INK4B) or p18 genes. We conclude that p16 super(INK4) mutations play a role in the formation of some NSCLCs, whereas the involvement of p15 super(INK4B) and p18 is uncommon.

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