Intranasal Vaccination Affords Localization and Persistence of Antigen-Specific CD8+ T Lymphocytes in the Female Reproductive Tract

Shailbala Singh; Kimberly S Schluns; Guojun Yang; Scott M Anthony; Michael A Barry; K. Jagannadha Sastry

doi:10.3390/vaccines4010007

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Intranasal Vaccination Affords Localization and Persistence of Antigen-Specific CD8+ T Lymphocytes in the Female Reproductive Tract

Journal article

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Intranasal Vaccination Affords Localization and Persistence of Antigen-Specific CD8+ T Lymphocytes in the Female Reproductive Tract

Shailbala Singh, Kimberly S Schluns, Guojun Yang, Scott M Anthony, Michael A Barry and K. Jagannadha Sastry

Vaccines (Basel), Vol.4(1), pp.7-7

03/17/2016

DOI: 10.3390/vaccines4010007

PMCID: PMC4810059

PMID: 26999228

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Abstract

Immunization strategies generating large numbers of antigen-specific T cells in the female reproductive tract (FRT) can provide barrier protection against sexually-transmitted pathogens, such as the human immunodeficiency virus (HIV) and human papillomaviruses (HPV). The kinetics and mechanisms of regulation of vaccine-induced adaptive T cell-mediated immune responses in FRT are less well defined. We present here evidence for intranasal delivery of the model antigen ovalbumin (OVA) along with alpha-galactosylceramide adjuvant as a protein vaccine to induce significantly higher levels of antigen-specific effector and memory CD8 + T cells in the FRT, relative to other systemic and mucosal tissues. Antibody blocking of the CXCR3 receptor significantly reduced antigen-specific CD8 + T cells subsequent to intranasal delivery of the protein vaccine suggesting an important role for the CXCR3 chemokine-receptor signaling for T cell trafficking. Further, intranasal vaccination with an adenoviral vector expressing OVA or HIV-1 envelope was as effective as intramuscular vaccination for generating OVA- or ENV-specific immunity in the FRT. These results support the application of the needle-free intranasal route as a practical approach to delivering protein as well as DNA/virus vector-based vaccines for efficient induction of effector and memory T cell immunity in the FRT.

CD8+ T cells

Female reproductive tract

intranasal immunization

Details

Title: Subtitle: Intranasal Vaccination Affords Localization and Persistence of Antigen-Specific CD8+ T Lymphocytes in the Female Reproductive Tract
Creators: Shailbala Singh - The University of Texas MD Anderson Cancer Center
Kimberly S Schluns - Immunology
Guojun Yang - The University of Texas MD Anderson Cancer Center
Scott M Anthony - The University of Texas Health Science Center at Houston
Michael A Barry - Mayo Clinic
K. Jagannadha Sastry - Immunology
Resource Type: Journal article
Publication Details: Vaccines (Basel), Vol.4(1), pp.7-7
DOI: 10.3390/vaccines4010007
PMID: 26999228
PMCID: PMC4810059
NLM abbreviation: Vaccines (Basel)
ISSN: 2076-393X
eISSN: 2076-393X
Publisher: MDPI
Language: English
Date published: 03/17/2016
Academic Unit: Pathology
Record Identifier: 9984183986802771

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