Intraoperative Detection and Removal of Microscopic Residual Sarcoma Using Wide-Field Imaging

Jeffrey K MITO; Rebecca D DODD; Jorge M FERRER; Yongbaek KIM; W David  Lee; Linda G GRIFFITH; Moungi G BAWENDI; David G KIRSCH; Brian E BRIGMAN; Chang-Lung LEE; William C EWARD; Lisa F MARSHALL; Kyle C CUNEO; Jessica E CARTER; Shalini RAMASUNDER

doi:10.1002/cncr.27458

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Intraoperative Detection and Removal of Microscopic Residual Sarcoma Using Wide-Field Imaging

Journal article

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Peer reviewed

Intraoperative Detection and Removal of Microscopic Residual Sarcoma Using Wide-Field Imaging

Jeffrey K MITO, Rebecca D DODD, Jorge M FERRER, Yongbaek KIM, W David Lee, Linda G GRIFFITH, Moungi G BAWENDI, David G KIRSCH, Brian E BRIGMAN, Chang-Lung LEE, …

Cancer, Vol.118(21), pp.5320-5330

2012

DOI: 10.1002/cncr.27458

PMCID: PMC3532657

PMID: 22437667

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Abstract

BACKGROUND: The goal of limb-sparing surgery for a soft tissue sarcoma of the extremity is to remove all malignant cells while preserving limb function. After initial surgery, microscopic residual disease in the tumor bed will cause a local recurrence in approximately 33% of patients with sarcoma. To help identify these patients, the authors developed an in vivo imaging system to investigate the suitability of molecular imaging for intraoperative visualization. METHODS: A primary mouse model of soft tissue sarcoma and a wide field-of-view imaging device were used to investigate a series of exogenously administered, near-infrared (NIR) fluorescent probes activated by cathepsin proteases for real-time intraoperative imaging. RESULTS: The authors demonstrated that exogenously administered cathepsin-activated probes can be used for image-guided surgery to identify microscopic residual NIR fluorescence in the tumor beds of mice. The presence of residual NIR fluorescence was correlated with microscopic residual sarcoma and local recurrence. The removal of residual NIR fluorescence improved local control. CONCLUSIONS: The authors concluded that their technique has the potential to be used for intraoperative image-guided surgery to identify microscopic residual disease in patients with cancer.

Dermatology

Tumors

Tumors of the skin and soft tissue. Premalignant lesions

Biological and medical sciences

Medical sciences

Details

Title: Subtitle: Intraoperative Detection and Removal of Microscopic Residual Sarcoma Using Wide-Field Imaging
Creators: Jeffrey K MITO - Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina, United States
Rebecca D DODD - Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina, United States
Jorge M FERRER - Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States
Yongbaek KIM - Department of Clinical Pathology, College of Veterinary Medicine, Seoul National University, Seoul, Korea, Republic of
W David Lee - David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States
Linda G GRIFFITH - Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States
Moungi G BAWENDI - Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States
David G KIRSCH - Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina, United States
Brian E BRIGMAN - Department of Orthopedic Surgery, Duke University Medical Center, Durham, North Carolina, United States
Chang-Lung LEE - Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina, United States
William C EWARD - Department of Orthopedic Surgery, Duke University Medical Center, Durham, North Carolina, United States
Lisa F MARSHALL - Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States
Kyle C CUNEO - Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina, United States
Jessica E CARTER - Department of Radiation Oncology, Duke University Medical Center, Durham, North Carolina, United States
Shalini RAMASUNDER - Department of Orthopedic Surgery, Duke University Medical Center, Durham, North Carolina, United States
Resource Type: Journal article
Publication Details: Cancer, Vol.118(21), pp.5320-5330
Publisher: Wiley-Blackwell
DOI: 10.1002/cncr.27458
PMID: 22437667
PMCID: PMC3532657
ISSN: 0008-543X
eISSN: 1097-0142
Language: English
Date published: 2012
Academic Unit: Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
Record Identifier: 9984094501202771

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