Journal article
Intrapericardial administration of adenovirus for gene transfer
American journal of physiology. Heart and circulatory physiology, Vol.272(1), pp.H310-H317
01/01/1997
DOI: 10.1152/ajpheart.1997.272.1.H310
PMID: 9038951
Abstract
Gene transfer to the heart has been accomplished with intravascular administration of adenoviral vectors into the pericardial sac, by increasing the duration of exposure to the adenovirus, would result in gene expression in the pericardium and perhaps myocardium and therefore might provide an alternative method to intravascular administration for gene transfer. We injected a replication-deficient adenovirus (average 1 x 10(12) particles/ml in 3% sucrose; 1 x 10(10) plaque forming units/ml containing cDNA encoding a nuclear-targeted bacterial beta-galactosidase into the pericardial sac of dogs. Samples of the pericardium and heart were examined for enzymatic activity of beta-galactosidase and after histochemical staining with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside. One day after injection of the adenovirus (1-3 ml), beta-galactosidase activity was highest in the parietal pericardium and left atrial tissue and lower in the right and left ventricles. Histochemical expression of the transgene was predominantly in the visceral pericardium of atria and ventricles and occasionally in the epicardial myocytes, arterioles, and venules. Pretreatment with doxycycline (5 mg) before adenovirus administration increased transgene activity in left ventricles. Thus adenovirus injected into the pericardial sac provides an effective method for gene transfer to the visceral and parietal pericardium over atria and ventricles.
Details
- Title: Subtitle
- Intrapericardial administration of adenovirus for gene transfer
- Creators
- Kathryn G Lamping - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242, USAC. David Rios - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242, USAJon A Chun - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242, USAHiroaki Ooboshi - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242, USABeverly L Davidson - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242, USADonald D Heistad - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242, USA
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Heart and circulatory physiology, Vol.272(1), pp.H310-H317
- DOI
- 10.1152/ajpheart.1997.272.1.H310
- PMID
- 9038951
- ISSN
- 0363-6135
- eISSN
- 1522-1539
- Language
- English
- Date published
- 01/01/1997
- Academic Unit
- Cardiovascular Medicine; Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984040461002771
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