Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice

Barun Das; Jashdeep Bhattacharjee; Preeti; Alaknanda Mishra; Kshama Jain; Srikanth Iyer; Ashwani Kesarwani; Parul Sahu; Prakriti Sinha; Perumal Nagarajan; Pramod Upadhyay

doi:10.3791/56018

Back

Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice

Journal article

Open access

Peer reviewed

Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice

Barun Das, Jashdeep Bhattacharjee, Preeti, Alaknanda Mishra, Kshama Jain, Srikanth Iyer, Ashwani Kesarwani, Parul Sahu, Prakriti Sinha, Perumal Nagarajan, …

Journal of Visualized Experiments, Vol.2018(132), e56018

02/10/2018

DOI: 10.3791/56018

PMCID: PMC5912392

PMID: 29553548

Files and links (1)

url

https://doi.org/10.3791/56018View

Published (Version of record) Open Access

Abstract

Partial hepatectomy is a versatile and reproducible method to study liver regeneration and the effect of cell based therapeutics in various pathological conditions. Partial hepatectomy also facilitates the increased engraftment and proliferation of transplanted cells by accelerating neovascularization and cell migration towards the liver. Here, we describe a simple protocol for performing 30% hepatectomy and transplantation of cells in the spleen of a non-obese diabetic/severe combined immunodeficient NOD.SCID (NOD.CB17- Prkdc scid /J) mouse. In this procedure, two small incisions are made. The first incision is to expose and resect the left lobe of the liver, and another small incision is made to expose the spleen for the intrasplenic transplantation of cells. This procedure does not require any specialized surgical skills, and it can be completed in 5-7 minutes with less stress and pain, faster recovery, and better survival. We have demonstrated the transplantation of hepatocytes isolated from a green fluorescent protein (GFP) expressing mouse (Transgenic C57BL/6-Tg (UBC-GFP) 30Scha/J), as well as hepatocyte like cells of human origin (NeoHep) in partially hepatectomized NOD.SCID mice.

Medicine

Details

Title: Subtitle: Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice
Creators: Barun Das - National Institute of Immunology
Jashdeep Bhattacharjee - National Institute of Immunology
Preeti - National Institute of Immunology
Alaknanda Mishra - National Institute of Immunology
Kshama Jain - National Institute of Immunology
Srikanth Iyer - National Institute of Immunology
Ashwani Kesarwani - National Institute of Immunology
Parul Sahu - National Institute of Immunology
Prakriti Sinha - National Institute of Immunology
Perumal Nagarajan - National Institute of Immunology
Pramod Upadhyay - National Institute of Immunology
Resource Type: Journal article
Publication Details: Journal of Visualized Experiments, Vol.2018(132), e56018
DOI: 10.3791/56018
PMID: 29553548
PMCID: PMC5912392
NLM abbreviation: J Vis Exp
ISSN: 1940-087X
eISSN: 1940-087X
Publisher: MyJoVE Corporation
Language: English
Date published: 02/10/2018
Academic Unit: Surgery
Record Identifier: 9985244581602771

Metrics

1 Record Views

2 Times Cited - Web of Science