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Intratracheal aerosolization of viral vectors to newborn pig airways
Journal article   Open access   Peer reviewed

Intratracheal aerosolization of viral vectors to newborn pig airways

Ashley L Cooney and Patrick L Sinn
BioTechniques, Vol.68(5), pp.235-239
05/2020
DOI: 10.2144/btn-2019-0150
PMCID: PMC7252491
PMID: 32141776
url
https://doi.org/10.2144/btn-2019-0150View
Published (Version of record) Open Access

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Abstract

Gene therapy for airway diseases requires efficient delivery of nucleic acids to the airways. In small animal models, gene delivery reagents are commonly delivered as a bolus dose. However, large animal models are often more relevant for the transition from preclinical studies to human trials. Aerosolizing viral vectors to the lungs of large animals can maximize anatomical distribution. Here, we describe a technique for aerosolization of viral vectors to the airways of newborn pigs. Briefly, a pig is anesthetized and intubated with an endotracheal tube, and a microsprayer is passed through the endotracheal tube. A fine mist is then sprayed into the distal trachea. Widespread and uniform distribution of transgene expression is critical for developing successful lung gene therapy treatments. METHOD SUMMARY Historically, achieving uniform distribution of a gene therapy reagent in the lungs has been challenging. Here, we describe an aerosolizing technique which can be used to achieve homogenous expression of a viral vector in newborn pig lungs. Briefly, pigs are sedated and intubated with an endotracheal tube, and a microsprayer is used to aerosolize a viral vector which results in its widespread distribution in the lungs.

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