Intravenous Human Umbilical Cord-Derived Mesenchymal Stromal Cell Administration in Models of Moderate and Severe Intracerebral Hemorrhage

Tanira Giara Mello; Paulo Henrique Rosado-de-Castro; Raquel Maria Pereira Campos; Juliana Ferreira Vasques; William Simoes Rangel-Junior; Raphael Santos de Almeida Rezende de Mattos; Teresa Puig-Pijuan; Bernd Uwe Foerster; Bianca Gutfilen; Sergio Augusto Lopes Souza; Johannes Boltze; Fernando Fernandes Paiva; Rosalia Mendez-Otero; Pedro Moreno Pimentel-Coelho

doi:10.1089/scd.2019.0176

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Intravenous Human Umbilical Cord-Derived Mesenchymal Stromal Cell Administration in Models of Moderate and Severe Intracerebral Hemorrhage

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Intravenous Human Umbilical Cord-Derived Mesenchymal Stromal Cell Administration in Models of Moderate and Severe Intracerebral Hemorrhage

Tanira Giara Mello, Paulo Henrique Rosado-de-Castro, Raquel Maria Pereira Campos, Juliana Ferreira Vasques, William Simoes Rangel-Junior, Raphael Santos de Almeida Rezende de Mattos, Teresa Puig-Pijuan, Bernd Uwe Foerster, Bianca Gutfilen, Sergio Augusto Lopes Souza, …

Stem cells and development, Vol.29(9), pp.586-598

05/01/2020

DOI: 10.1089/scd.2019.0176

PMID: 32160799

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Abstract

Intracerebral hemorrhage (ICH) is as a life-threatening condition that can occur in young adults, often causing long-term disability. Recent preclinical data suggest mesenchymal stromal cell (MSC)-based therapies as promising options to minimize brain damage after ICH. However, therapeutic evidence and mechanistic insights are still limited, particularly when compared with other disorders such as ischemic stroke. Herein, we employed a model of collagenase-induced ICH in young adult rats to investigate the potential therapeutic effects of an intravenous injection of human umbilical cord Wharton's jelly-derived MSCs (hUC-MSCs). Two doses of collagenase were used to cause moderate or severe hemorrhages. Magnetic resonance imaging showed that animals treated with hUC-MSCs after moderate ICH had smaller residual hematoma volumes than vehicle-treated rats, whereas the cell therapy failed to decrease the hematoma volume in animals with a severe ICH. Functional assessments (rotarod and elevated body swing tests) were performed for up to 21 days after ICH. Enduring neurological impairments were seen only in animals subjected to severe ICH, but the cell therapy did not induce statistically significant improvements in the functional recovery. The biodistribution of Technetium-99m-labeled hUC-MSCs was also evaluated, showing that most cells were found in organs such as the spleen and lungs 24 h after transplantation. Nevertheless, it was possible to detect a weak signal in the brain, which was higher in the ipsilateral hemisphere of rats subjected to a severe ICH. These data indicate that hUC-MSCs have moderately beneficial effects in cases of less severe brain hemorrhages in rats by decreasing the residual hematoma volume, and that optimization of the therapy is still necessary.

Cell & Tissue Engineering

Cell Biology

Hematology

Life Sciences & Biomedicine

Medicine, Research & Experimental

Research & Experimental Medicine

Science & Technology

Transplantation

Details

Title: Subtitle: Intravenous Human Umbilical Cord-Derived Mesenchymal Stromal Cell Administration in Models of Moderate and Severe Intracerebral Hemorrhage
Creators: Tanira Giara Mello - Nuclear Engineering Institute
Paulo Henrique Rosado-de-Castro - National Institute of Science and Technology in Regenerative Medicine
Raquel Maria Pereira Campos - Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Av Carlos Chagas Filho 373,Bloco G,Sala G2-001, BR-21941902 Rio De Janeiro, RJ, Brazil
Juliana Ferreira Vasques - Universidade Federal do Rio de Janeiro
William Simoes Rangel-Junior - Universidade Federal do Rio de Janeiro
Raphael Santos de Almeida Rezende de Mattos - Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Av Carlos Chagas Filho 373,Bloco G,Sala G2-001, BR-21941902 Rio De Janeiro, RJ, Brazil
Teresa Puig-Pijuan - National Institute of Science and Technology in Regenerative Medicine
Bernd Uwe Foerster - Universidade de São Paulo
Bianca Gutfilen - Universidade Federal do Rio de Janeiro
Sergio Augusto Lopes Souza - Univ Fed Rio de Janeiro, Fac Med, Dept Radiol, Rio De Janeiro, Brazil
Johannes Boltze - University of Warwick
Fernando Fernandes Paiva - Universidade de São Paulo
Rosalia Mendez-Otero - Universidade Federal do Rio de Janeiro
Pedro Moreno Pimentel-Coelho - National Institute of Science and Technology in Regenerative Medicine
Resource Type: Journal article
Publication Details: Stem cells and development, Vol.29(9), pp.586-598
DOI: 10.1089/scd.2019.0176
PMID: 32160799
NLM abbreviation: Stem Cells Dev
ISSN: 1547-3287
eISSN: 1557-8534
Publisher: Mary Ann Liebert, Inc
Number of pages: 13
Grant note: 88887.136364/2017-00; PROBITEC 2262/2012 -23038.004161/2018-88 / Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) 19/2016 / Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ); Fundacao Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio De Janeiro (FAPERJ) Departamento de Ciencia e Tecnologia (DECIT) do Ministerio da Saude 402319/2013-3 / Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq); Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ) Instituto Nacional de Ciencia e Tecnologia em Medicina Regenerativa
Language: English
Date published: 05/01/2020
Academic Unit: Radiology
Record Identifier: 9985143125902771

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