Journal article
Intravenous and intra-arterial administration of bone marrow mononuclear cells after focal cerebral ischemia: Is there a difference in biodistribution and efficacy?
Stem cell research, Vol.9(1), pp.1-8
07/01/2012
DOI: 10.1016/j.scr.2012.02.002
PMID: 22445868
Abstract
Intravascular delivery of cells has been increasingly used in stroke models and clinical trials. We compared the biodistribution and therapeutic effects of bone marrow mononuclear cells (BMMCs) delivered by intra-arterial (IA) or intravenous (IV) injection after cortical ischemia. For the biodistribution analyses, BMMCs were labeled with (99m)Technetium (Tc-99m). At 2 h, gamma-well counting of the brain and of the other organs evaluated did not show differences between the non-ischemic and ischemic groups or between injection routes, and the organs with the highest uptake were the liver and lungs, with low uptake in the brain. At 24 h, the liver maintained the highest activity, and a marked decrease was seen in pulmonary uptake in all groups. At this time point, although the activity in the brain remained low, the lesioned hemisphere showed greater homing than the contralateral hemisphere, for both the IV and IA ischemic groups. Histological analysis by CellTrace labeling indicated similar homing between both routes in the pen-infarct region 24 h after transplantation and functional recovery was observed in both groups up to 11 weeks after the lesion. In conclusion, transplantation of BMMCs by IA or IV routes may lead to similar brain homing and therapeutic efficacy after experimental stroke. (C) 2012 Elsevier B.V. All rights reserved.
Details
- Title: Subtitle
- Intravenous and intra-arterial administration of bone marrow mononuclear cells after focal cerebral ischemia: Is there a difference in biodistribution and efficacy?
- Creators
- Andreia Vasconcelos-dos-Santos - Universidade Federal do Rio de JaneiroPaulo Henrique Rosado-de-Castro - Universidade Federal do Rio de JaneiroSergio Augusto Lopes de Souza - Univ Fed Rio de Janeiro, Dept Radiol, Hosp Univ Clementino Fraga Filho, BR-21941902 Rio De Janeiro, RJ, BrazilJuliana da Costa SilvaAlane Bernardo Ramos - Universidade Federal do Rio de JaneiroGabriel Rodriguez de Freitas - Universidade Federal do Rio de JaneiroLea Mirian Barbosa da Fonseca - Univ Fed Rio de Janeiro, Dept Radiol, Hosp Univ Clementino Fraga Filho, BR-21941902 Rio De Janeiro, RJ, BrazilBianca Gutfilen - Universidade Federal do Rio de JaneiroRosalia Mendez-Otero - Universidade Federal do Rio de Janeiro
- Resource Type
- Journal article
- Publication Details
- Stem cell research, Vol.9(1), pp.1-8
- DOI
- 10.1016/j.scr.2012.02.002
- PMID
- 22445868
- NLM abbreviation
- Stem Cell Res
- ISSN
- 1873-5061
- eISSN
- 1876-7753
- Publisher
- Elsevier
- Number of pages
- 8
- Language
- English
- Date published
- 07/01/2012
- Academic Unit
- Radiology
- Record Identifier
- 9985143552302771
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