Journal article
Intravesical Dimethyl Sulfoxide Inhibits Acute and Chronic Bladder Inflammation in Transgenic Experimental Autoimmune Cystitis Models
Journal of biomedicine & biotechnology, Vol.2011, pp.937061-9
01/01/2011
DOI: 10.1155/2011/937061
PMCID: PMC2989383
PMID: 21113298
Abstract
New animal models are greatly needed in interstitial cystitis/painful bladder syndrome (IC/PBS) research. We recently developed a novel transgenic cystitis model (URO-OVA mice) that mimics certain key aspects of IC/PBS pathophysiology. This paper aimed to determine whether URO-OVA cystitis model was responsive to intravesical dimethyl sulfoxide (DMSO) and if so identify the mechanisms of DMSO action. URO-OVA mice developed acute cystitis upon adoptive transfer of OVA-specific OT-I splenocytes. Compared to PBS-treated bladders, the bladders treated with 50% DMSO exhibited markedly reduced bladder histopathology and expression of various inflammatory factor mRNAs. Intravesical DMSO treatment also effectively inhibited bladder inflammation in a spontaneous chronic cystitis model (URO-OVA/OT-I mice). Studies further revealed that DMSO could impair effector T cells in a dose-dependent manner in vitro. Taken together, our results suggest that intravesical DMSO improves the bladder histopathology of IC/PBS patients because of its ability to interfere with multiple inflammatory and bladder cell types.
Details
- Title: Subtitle
- Intravesical Dimethyl Sulfoxide Inhibits Acute and Chronic Bladder Inflammation in Transgenic Experimental Autoimmune Cystitis Models
- Creators
- Ronald Kim - Northwestern UniversityWujiang Liu - Univ Iowa, Dept Urol, Iowa City, IA 52242 USAXiaohong Chen - University of IowaKarl J. Kreder - Univ Iowa, Dept Urol, Iowa City, IA 52242 USAYi Luo - Univ Iowa, Dept Urol, Iowa City, IA 52242 USA
- Resource Type
- Journal article
- Publication Details
- Journal of biomedicine & biotechnology, Vol.2011, pp.937061-9
- DOI
- 10.1155/2011/937061
- PMID
- 21113298
- PMCID
- PMC2989383
- NLM abbreviation
- J Biomed Biotechnol
- ISSN
- 1110-7243
- eISSN
- 1110-7251
- Publisher
- Hindawi Publishing Group
- Number of pages
- 9
- Grant note
- U01DK082344-020005 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA W81XWH-04-1-0070 / DOD; United States Department of Defense U01DK082344 / NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK)
- Language
- English
- Date published
- 01/01/2011
- Academic Unit
- Obstetrics and Gynecology; Urology
- Record Identifier
- 9984319979902771
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