Journal article
Intrinsic interactions between alkaline earth metal ions and peptides: a gas-phase study
Journal of the American Chemical Society, Vol.112(11), pp.4110-4120
1990
DOI: 10.1021/ja00167a003
Abstract
The gas-phase chemistry of (M – H = Cat)+ complexes formed between alkaline-earth metal ions and simple peptides is reported. The preferred sites of metal ion complexation are determined from collision-induced decompositions of the complexes that occur in the first field-free region of a normal-geometry mass spectrometer. Mechanisms for formation of N-terminal a-and c-sequence ions primarily require the (M – H = Cat)+ complexes to contain the alkaline-earth metal ion coordinated to a deprotonated amide nitrogen. The C-terminal y-sequence ions appear to arise exclusively from (M – H = Cat)+ ions that contain either a deprotonated and cationized C-terminal carboxylate or an amide. The C-terminal z-sequence ions, however, can arise from deprotonation and coordination of the metal ion either to a C-terminal carboxylate or amide or to an intervening deprotonated amide group. Changes in fragmentation patterns with increasing size of alkaline-earth metal ion, with substitution of the C-terminal carboxylate for a C-terminal amide, and with changes in side-chain structure suggest that interactions with the metal ions also may be via more extensive intramolecular complexes that are affected by coordination geometry of the metal ion. The collision-induced decompositions of the peptide–alkaline-earth ion complexes are analytically useful for providing peptide sequence information.
Details
- Title: Subtitle
- Intrinsic interactions between alkaline earth metal ions and peptides: a gas-phase study
- Creators
- Lynn M TeeschJeanette Adams
- Resource Type
- Journal article
- Publication Details
- Journal of the American Chemical Society, Vol.112(11), pp.4110-4120
- Publisher
- American Chemical Society
- DOI
- 10.1021/ja00167a003
- ISSN
- 0002-7863
- eISSN
- 1520-5126
- Language
- English
- Date published
- 1990
- Academic Unit
- Medicine Administration; Core Research Facilities
- Record Identifier
- 9984622053002771
Metrics
1 Record Views