Journal article
Intrinsically disordered regions are not sufficient to direct the compartmental localization of nucleolar proteins in the nucleus
PLoS biology, Vol.21(11), e3002378
11/09/2023
DOI: 10.1371/journal.pbio.3002378
PMCID: PMC10662738
PMID: 37943867
Appears in UI Libraries Support Open Access
Abstract
The nucleolus is a non-membrane bound organelle central to ribosome biogenesis. The nucleolus contains a mix of proteins and RNA and has 3 known nucleolar compartments: the fibrillar center (FC), the dense fibrillar component (DFC), and the granular component (GC). The spatial organization of the nucleolus is influenced by the phase separation properties of nucleolar proteins, the presence of RNA, protein modification, and cellular activity. Many nucleolar proteins appear to concentrate within the borders of the compartments. We investigated whether the intrinsically disordered regions from several proteins provided the information needed to establish specific compartment localization using Xenopus laevis oocytes. For the proteins we tested, the disordered regions were not sufficient to direct specific domain localization and appear dispensable with respect to compartmentalization. Among the proteins that colocalize to the DFC are the quartet that comprise the box H/ACA pseudouridylation complex. In contrast to the insufficiency of IDRs to direct compartment localization, we found that the DFC accumulation of 2 box H/ACA proteins, Gar1 and Nhp2, was disrupted by mutations that were previously shown to reduce their ability to join the box H/ACA complex. Using a nanobody to introduce novel binding to a different DFC localized protein, we restored the localization of the mutated forms of Gar1 and Nhp2.
Details
- Title: Subtitle
- Intrinsically disordered regions are not sufficient to direct the compartmental localization of nucleolar proteins in the nucleus
- Creators
- Emily D Lavering - University of IowaMaunika Gandhamaneni - University of IowaDaniel L Weeks - University of Iowa
- Resource Type
- Journal article
- Publication Details
- PLoS biology, Vol.21(11), e3002378
- DOI
- 10.1371/journal.pbio.3002378
- PMID
- 37943867
- PMCID
- PMC10662738
- NLM abbreviation
- PLoS Biol
- ISSN
- 1545-7885
- eISSN
- 1545-7885
- Publisher
- PLOS
- Grant note
- DOI: 10.13039/100000057, name: NIGMS, award: T32 GM067795; DOI: 10.13039/100008893, name: University of Iowa, award: ICRU undergraduate fellowship; DOI: 10.13039/100000057, name: NIGMS, award: R01 GM124063; DOI: 10.13039/100008893, name: University of Iowa, award: Iowa Center for Aging
- Language
- English
- Date published
- 11/09/2023
- Academic Unit
- Stead Family Department of Pediatrics; Biochemistry and Molecular Biology
- Record Identifier
- 9984511948502771
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