Inventory and phylogenetic analysis of meiotic genes in monogonont rotifers

Sara J Hanson; Andrew M Schurko; Bette Hecox-Lea; David B Mark Welch; Claus-Peter Stelzer; John M Logsdon Jr

doi:10.1093/jhered/est011

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Inventory and phylogenetic analysis of meiotic genes in monogonont rotifers

Journal article

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Inventory and phylogenetic analysis of meiotic genes in monogonont rotifers

Sara J Hanson, Andrew M Schurko, Bette Hecox-Lea, David B Mark Welch, Claus-Peter Stelzer and John M Logsdon Jr

The Journal of heredity, Vol.104(3), pp.357-370

05/2013

DOI: 10.1093/jhered/est011

PMCID: PMC3622358

PMID: 23487324

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Abstract

A long-standing question in evolutionary biology is how sexual reproduction has persisted in eukaryotic lineages. As cyclical parthenogens, monogonont rotifers are a powerful model for examining this question, yet the molecular nature of sexual reproduction in this lineage is currently understudied. To examine genes involved in meiosis, we generated partial genome assemblies for 2 distantly related monogonont species, Brachionus calyciflorus and B. manjavacas. Here we present an inventory of 89 meiotic genes, of which 80 homologs were identified and annotated from these assemblies. Using phylogenetic analysis, we show that several meiotic genes have undergone relatively recent duplication events that appear to be specific to the monogonont lineage. Further, we compare the expression of "meiosis-specific" genes involved in recombination and all annotated copies of the cell cycle regulatory gene CDC20 between obligate parthenogenetic (OP) and cyclical parthenogenetic (CP) strains of B. calyciflorus. We show that "meiosis-specific" genes are expressed in both CP and OP strains, whereas the expression of one of the CDC20 genes is specific to cyclical parthenogenesis. The data presented here provide insights into mechanisms of cyclical parthenogenesis and establish expectations for studies of obligate asexual relatives of monogononts, the bdelloid rotifer lineage.

DNA Replication

Animals

Cell Cycle Proteins - genetics

Chromosomes - genetics

Expressed Sequence Tags

Gene Expression Regulation

Meiosis - genetics

Parthenogenesis - genetics

Phylogeny

Rotifera - genetics

Details

Title: Subtitle: Inventory and phylogenetic analysis of meiotic genes in monogonont rotifers
Creators: Sara J Hanson - Department of Biology, University of Iowa, Iowa City, IA 52242, USA
Andrew M Schurko
Bette Hecox-Lea
David B Mark Welch
Claus-Peter Stelzer
John M Logsdon Jr
Resource Type: Journal article
Publication Details: The Journal of heredity, Vol.104(3), pp.357-370
DOI: 10.1093/jhered/est011
PMID: 23487324
PMCID: PMC3622358
ISSN: 0022-1503
eISSN: 1465-7333
Grant note: T32 GM008629 / NIGMS NIH HHS 5R01GM079484 / NIGMS NIH HHS
Language: English
Date published: 05/2013
Academic Unit: Biology
Record Identifier: 9984217535802771

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