Investigation of Break-Induced Replication in Yeast

Beth Osia; Rajula Elango; Juraj Kramara; Steven A Roberts; Anna Malkova

doi:10.1007/978-1-0716-0644-5_22

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Journal article

Investigation of Break-Induced Replication in Yeast

Beth Osia, Rajula Elango, Juraj Kramara, Steven A Roberts and Anna Malkova

Methods in molecular biology (Clifton, N.J.), Vol.2153, pp.307-328

2021

DOI: 10.1007/978-1-0716-0644-5_22

PMCID: PMC9041317

PMID: 32840789

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https://www.ncbi.nlm.nih.gov/pmc/articles/9041317View

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Abstract

Repair of double-strand DNA breaks (DSBs) is important for preserving genomic integrity and stability. Break-induced replication (BIR) is a mechanism aimed to repair one-ended double-strand DNA breaks, similar to those formed by replication fork collapse or by telomere erosion. Unlike S-phase replication, BIR is carried out by a migrating DNA bubble and is associated with conservative inheritance of newly synthesized DNA. This unusual DNA synthesis leads to high level of mutagenesis and chromosomal rearrangements during BIR. Here, we focus on several genetic and molecular methods to investigate BIR using our system in yeast Saccharomyces cerevisiae where BIR is initiated by a site-specific DNA break, and the repair involves two copies of chromosome III.

Chromosomes, Fungal - genetics

Cytidine Deaminase - metabolism

DNA Breaks, Double-Stranded

DNA Replication

Mutation

Recombinational DNA Repair

Saccharomyces cerevisiae - genetics

Saccharomyces cerevisiae - physiology

Saccharomyces cerevisiae Proteins - metabolism

Details

Title: Subtitle: Investigation of Break-Induced Replication in Yeast
Creators: Beth Osia - Department of Biology, University of Iowa, Iowa City, IA, USA
Rajula Elango - Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA
Juraj Kramara - Department of Biology, University of Iowa, Iowa City, IA, USA
Steven A Roberts - School of Molecular Biosciences, Center for Reproductive Biology, Washington State University, Pullman, WA, USA
Anna Malkova - Department of Biology, University of Iowa, Iowa City, IA, USA. anna-malkova@uiowa.edu
Resource Type: Journal article
Publication Details: Methods in molecular biology (Clifton, N.J.), Vol.2153, pp.307-328
DOI: 10.1007/978-1-0716-0644-5_22
PMID: 32840789
PMCID: PMC9041317
NLM abbreviation: Methods Mol Biol
eISBN: 1071606441; 9781071606445
ISSN: 1064-3745
eISSN: 1940-6029
Grant note: R21 ES030307 / NIEHS NIH HHS R35 GM127006 / NIGMS NIH HHS R01 CA218112 / NCI NIH HHS R03 ES029306 / NIEHS NIH HHS
Language: English
Date published: 2021
Academic Unit: Stead Family Department of Pediatrics; Biology; Infectious Disease (Pediatrics)
Record Identifier: 9984217525202771

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