Journal article
Investigation of Break-Induced Replication in Yeast
Methods in enzymology, Vol.601, pp.161-203
2018
DOI: 10.1016/bs.mie.2017.12.010
PMID: 29523232
Abstract
Break-induced replication (BIR) is an important mechanism aimed to repair one-ended double-strand DNA breaks. BIR is initiated by invasion of a broken DNA end into a homologous template followed by DNA synthesis that can proceed for hundreds of kilobases to the end of the chromosome. Unlike S-phase replication, BIR is carried out by a migrating DNA bubble and is associated with conservative inheritance of newly synthesized DNA. The unusual mode of DNA synthesis during BIR leads to an increased level of genetic instabilities including increased mutagenesis and chromosomal rearrangements. Here, we describe our experimental system in yeast Saccharomyces cerevisiae where BIR is initiated by a site-specific DNA break and where the repair involves two copies of chromosome III. This system allows investigation of BIR using genetic and molecular biology approaches, and can be used for characterization of the BIR mechanism, roles of individual proteins in BIR, and for the analysis of genetic instabilities associated with BIR.
Details
- Title: Subtitle
- Investigation of Break-Induced Replication in Yeast
- Creators
- Rajula Elango - University of Iowa, Iowa City, IA, United StatesZachary Kockler - University of Iowa, Iowa City, IA, United StatesLiping Liu - University of Iowa, Iowa City, IA, United StatesAnna Malkova - University of Iowa, Iowa City, IA, United States. Electronic address: anna-malkova@uiowa.edu
- Resource Type
- Journal article
- Publication Details
- Methods in enzymology, Vol.601, pp.161-203
- DOI
- 10.1016/bs.mie.2017.12.010
- PMID
- 29523232
- ISSN
- 0076-6879
- eISSN
- 1557-7988
- Grant note
- R01 GM084242 / NIGMS NIH HHS
- Language
- English
- Date published
- 2018
- Academic Unit
- Biology
- Record Identifier
- 9984217534902771
Metrics
6 Record Views