Investigation of the Developmental Requirements of Drosophila HP1 and Insulator Protein Partner, HIPP1

Steve E Glenn; Pamela K Geyer

doi:10.1534/g3.118.200705

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Investigation of the Developmental Requirements of Drosophila HP1 and Insulator Protein Partner, HIPP1

Journal article

Open access

Investigation of the Developmental Requirements of Drosophila HP1 and Insulator Protein Partner, HIPP1

Steve E Glenn and Pamela K Geyer

G3 : genes - genomes - genetics, Vol.9(2), pp.345-357

02/07/2019

DOI: 10.1534/g3.118.200705

PMCID: PMC6385973

PMID: 30514714

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Abstract

Drosophila Suppressor of Hairy-wing [Su(Hw)] is a multifunctional zinc finger DNA binding protein. Transcriptional regulation by Su(Hw) is essential in the ovary and testis, where Su(Hw) functions primarily as a repressor. Recently, the HP1a and Insulator Partner Protein 1 (HIPP1) was found to extensively co-localize with Su(Hw) and other insulator binding proteins in euchromatic regions of the genome, and with Heterochromatin Protein 1a (HP1a) in heterochromatic regions. As HIPP1 is the homolog of the human co-repressor Chromodomain Y-Like (CDYL), we tested its requirement in establishing transcriptional repression in flies. To this end, we generated multiple null alleles and a tagged derivative of the endogenous gene ( ), using CRISPR mutagenesis. We show that HIPP1 is a widely expressed nuclear protein that is dispensable for viability, as well as female and male fertility. We find that HIPP1 and HP1a display minimum co-localization in interphase cells, and HP1a-dependent transcriptional repression of several reporter genes is HIPP1-independent, indicating that HIPP1 is not essential for HP1a-dependent heterochromatin formation. Despite Su(Hw) having a major role in promoting HIPP1 occupancy in euchromatin, we show that HIPP1 is dispensable for the transcriptional and insulator functions of Su(Hw), indicating that HIPP1 is not a critical Su(Hw) cofactor. Further studies are needed to clarify the role of HIPP1 in Drosophila development.

Animals

Carrier Proteins - genetics

Carrier Proteins - metabolism

Drosophila melanogaster

Drosophila Proteins - genetics

Drosophila Proteins - metabolism

Female

Fertility - genetics

Gene Expression Regulation, Developmental

Heterochromatin - genetics

Insulator Elements

Male

Repressor Proteins - genetics

Repressor Proteins - metabolism

Details

Title: Subtitle: Investigation of the Developmental Requirements of Drosophila HP1 and Insulator Protein Partner, HIPP1
Creators: Steve E Glenn - Molecular Medicine Program
Pamela K Geyer - University of Iowa
Resource Type: Journal article
Publication Details: G3 : genes - genomes - genetics, Vol.9(2), pp.345-357
DOI: 10.1534/g3.118.200705
PMID: 30514714
PMCID: PMC6385973
NLM abbreviation: G3 (Bethesda)
ISSN: 2160-1836
eISSN: 2160-1836
Grant note: R01 GM042539 / NIGMS NIH HHS T32 GM082729 / NIGMS NIH HHS P30 CA086862 / NCI NIH HHS
Language: English
Date published: 02/07/2019
Academic Unit: Obstetrics and Gynecology; Biochemistry and Molecular Biology
Record Identifier: 9984293091002771

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