Journal article
Investigation of the Properties of Non-gypsy Suppressor of Hairy-wing-Binding Sites
Genetics (Austin), Vol.179(3), pp.1263-1273
07/2008
DOI: 10.1534/genetics.108.087254
PMCID: PMC2475731
PMID: 18562648
Abstract
Insulators define interactions between transcriptional control elements in eukaryotic genomes. The
gypsy
insulator found in the
gypsy
retrovirus binds the zinc-finger Suppressor of Hairy-wing [Su(Hw)] protein that associates with hundreds of non-
gypsy
regions throughout the Drosophila genome. Models of insulator function predict that the
gypsy
insulator forms chromatin loop domains through interactions with endogenous Su(Hw) insulators (SIs) to limit the action of transcriptional control elements. Here we study SI 62D and show that interactions occur between two SI 62D elements, but not between SI 62D and the
gypsy
insulator, limiting the scope of genomic
gypsy
insulator interactions. Enhancer blocking by SI 62D requires fewer Su(Hw)-binding sites than needed for
gypsy
insulator function, with these target regions having distinct zinc-finger requirements for
in vivo
Su(Hw) association. These observations led to an investigation of the role of the Su(Hw) zinc-finger domain in insulator function. Using a combination of
in vitro
and
in vivo
studies, we find that this domain makes sequence-dependent and -independent contributions to
in vivo
chromosome association, but is not essential for enhancer or silencer blocking. These studies extend our understanding of the properties of Su(Hw) and the endogenous genomic regions to which this protein localizes.
Details
- Title: Subtitle
- Investigation of the Properties of Non-gypsy Suppressor of Hairy-wing-Binding Sites
- Creators
- Emily J Kuhn-Parnell - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242Cecilia Helou - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242David J Marion - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242Brian L Gilmore - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242Timothy J Parnell - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242Marc S Wold - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242Pamela K Geyer - Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- Genetics (Austin), Vol.179(3), pp.1263-1273
- Publisher
- Genetics Society of America
- DOI
- 10.1534/genetics.108.087254
- PMID
- 18562648
- PMCID
- PMC2475731
- ISSN
- 0016-6731
- eISSN
- 1943-2631
- Language
- English
- Date published
- 07/2008
- Academic Unit
- Radiation Oncology; Obstetrics and Gynecology; Biochemistry and Molecular Biology
- Record Identifier
- 9984025297602771
Metrics
22 Record Views