Involvement of A1 adenosine receptors and neural pathways in adenosine-induced bronchoconstriction in mice

Xiaoyang Hua; Christopher J Erikson; Kelly D Chason; Craig N Rosebrock; Deepak A Deshpande; Raymond B Penn; Stephen L Tilley

doi:10.1152/ajplung.00058.2007

Back

Involvement of A1 adenosine receptors and neural pathways in adenosine-induced bronchoconstriction in mice

Journal article

Peer reviewed

Involvement of A1 adenosine receptors and neural pathways in adenosine-induced bronchoconstriction in mice

Xiaoyang Hua, Christopher J Erikson, Kelly D Chason, Craig N Rosebrock, Deepak A Deshpande, Raymond B Penn and Stephen L Tilley

American journal of physiology. Lung cellular and molecular physiology, Vol.293(1), pp.L25-32

07/2007

DOI: 10.1152/ajplung.00058.2007

PMID: 17468137

View Online

Abstract

High levels of adenosine can be measured from the lungs of asthmatics, and it is well recognized that aerosolized 5'AMP, the precursor of adenosine, elicits robust bronchoconstriction in patients with this disease. Characterization of mice with elevated adenosine levels secondary to the loss of adenosine deaminase (ADA) expression, the primary metabolic enzyme for adenosine, further support a role for this ubiquitous mediator in the pathogenesis of asthma. To begin to identify pathways by which adenosine can alter airway tone, we examined adenosine-induced bronchoconstriction in four mouse lines, each lacking one of the receptors for this nucleoside. We show, using direct measures of airway mechanics, that adenosine can increase airway resistance and that this increase in resistance is mediated by binding the A(1) receptor. Further examination of this response using pharmacologically, surgically, and genetically manipulated mice supports a model in which adenosine-induced bronchoconstriction occurs indirectly through the activation of sensory neurons.

Adenosine - analogs & derivatives

Adenosine - pharmacology

Adenosine-5'-(N-ethylcarboxamide) - pharmacology

Anesthesia

Animals

Bronchoconstriction - drug effects

In Vitro Techniques

Mice

Mice, Inbred C57BL

Muscle Contraction - drug effects

Muscle, Smooth - cytology

Muscle, Smooth - drug effects

Neural Pathways - metabolism

Receptor, Adenosine A1 - metabolism

Respiratory System - cytology

Respiratory System - drug effects

Vagotomy

Details

Title: Subtitle: Involvement of A1 adenosine receptors and neural pathways in adenosine-induced bronchoconstriction in mice
Creators: Xiaoyang Hua - University of North Carolina at Chapel Hill
Christopher J Erikson - University of North Carolina
Kelly D Chason - University of North Carolina
Craig N Rosebrock - University of North Carolina
Deepak A Deshpande - Wake Forest University
Raymond B Penn - Wake Forest University
Stephen L Tilley - Pulmonary
Resource Type: Journal article
Publication Details: American journal of physiology. Lung cellular and molecular physiology, Vol.293(1), pp.L25-32
DOI: 10.1152/ajplung.00058.2007
PMID: 17468137
ISSN: 1040-0605
eISSN: 1522-1504
Grant note: HL-071802 / NHLBI NIH HHS K99-HL-087560 / NHLBI NIH HHS HL-58506 / NHLBI NIH HHS
Language: English
Date published: 07/2007
Academic Unit: Otolaryngology
Record Identifier: 9984312250302771

Metrics

3 Record Views

32 Times Cited - Web of Science