Journal article
Involvement of ABC-transporters and acyltransferase 1 in intracellular cholesterol-mediated autophagy in bovine alveolar macrophages in response to the Bacillus Calmette-Guerin (BCG) infection
BMC immunology, Vol.21(1), p.26
05/12/2020
DOI: 10.1186/s12865-020-00356-x
PMCID: PMC7216371
PMID: 32397995
Abstract
Background: Understanding pathogenic mechanisms is imperative for developing novel treatment to the tuberculosis, an important public health burden worldwide. Recent studies demonstrated that host cholesterol levels have implications in the establishment of Mycobacterium tuberculosis (M. tuberculosis, Mtb) infection in host cells, in which the intracellular cholesterol-mediated ATP-binding cassette transporters (ABC-transporters) and cholesterol acyltransferase1 (ACAT1) exhibited abilities to regulate macrophage autophagy induced by Mycobacterium bovis bacillus Calmette-Guérin (BCG). Results: The results showed that a down-regulated expression of the ABC-transporters and ACAT1 in primary bovine alveolar macrophages (AMs) and murine RAW264.7 cells in response to a BCG infection. The inhibited expression of ABC-transporters and ACAT1 was associated with the reduction of intracellular free cholesterol, which in turn induced autophagy in macrophages upon to the Mycobacterial infection. These results strongly suggest an involvement of ABC-transporters and ACAT1 in intracellular cholesterol-mediated autophagy in AMs in response to BCG infection. Conclusion: This study thus provides an insight into into a mechanism by which the cholesterol metabolism regulated the autophagy in macrophages in response to mycobacterial infections.
Details
- Title: Subtitle
- Involvement of ABC-transporters and acyltransferase 1 in intracellular cholesterol-mediated autophagy in bovine alveolar macrophages in response to the Bacillus Calmette-Guerin (BCG) infection
- Creators
- Jinrui Xu - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Yinchuan, China Yinchuan, 750021 Ningxia ChinaYanbing Zhou - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Yinchuan, China Yinchuan, 750021 Ningxia ChinaYi Yang - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Yinchuan, China Yinchuan, 750021 Ningxia ChinaCuiping Lv - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Yinchuan, China Yinchuan, 750021 Ningxia ChinaXiaoming Liu - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Yinchuan, China Yinchuan, 750021 Ningxia ChinaYujiong Wang - Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Yinchuan, China Yinchuan, 750021 Ningxia China
- Resource Type
- Journal article
- Publication Details
- BMC immunology, Vol.21(1), p.26
- DOI
- 10.1186/s12865-020-00356-x
- PMID
- 32397995
- PMCID
- PMC7216371
- NLM abbreviation
- BMC Immunol
- ISSN
- 1471-2172
- eISSN
- 1471-2172
- Publisher
- BioMed Central
- Grant note
- 2019AAC03010 / ; 2017BN04 / ; 31772710; 31760733; 31960700; 31960712 / ;
- Language
- English
- Date published
- 05/12/2020
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984288825702771
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