Involvement of neurotrophin-4/5 in regeneration of the periodontal Ruffini endings at the early stage

Shahiqul Jabbar; Fumiko Harada; Megumi Aita; Megumi Ohishi; Isao Saito; Yoshiro Kawano; Akiko Suzuki; Kayoko Nozawa-Inoue; Takeyasu Maeda

doi:10.1002/cne.21256

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Involvement of neurotrophin-4/5 in regeneration of the periodontal Ruffini endings at the early stage

Journal article

Peer reviewed

Involvement of neurotrophin-4/5 in regeneration of the periodontal Ruffini endings at the early stage

Shahiqul Jabbar, Fumiko Harada, Megumi Aita, Megumi Ohishi, Isao Saito, Yoshiro Kawano, Akiko Suzuki, Kayoko Nozawa-Inoue and Takeyasu Maeda

Journal of comparative neurology (1911), Vol.501(3), pp.400-412

03/20/2007

DOI: 10.1002/cne.21256

PMID: 17245704

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Abstract

Little is known about the role of neurotrophin-4/5 (NT-4/5) in the regeneration of mechanoreceptors. Therefore, the present study examined the regeneration process of Ruffini endings in the periodontal ligament in nt-4/5-deficient and wildtype mice following transection of the inferior alveolar nerve by immunohistochemistry for protein gene product 9.5 (PGP 9.5), a general neuronal marker, and by computer-assisted quantitative image analysis. Furthermore, rescue experiments by a continuous administration of recombinant NT-4/5 were performed and analyzed quantitatively. At postoperative day 3 (PO 3d), almost all PGP 9.5-positive neural elements had disappeared; they began to appear in both types of animals at PO 7d. At PO 10d, almost all nerve fibers showed a beaded appearance, with fewer ramifications in both types of mice. Although the regeneration proceeded in the wildtype, a major population of the periodontal Ruffini endings continued to display smooth outlines at PO 28d in the nt-4/5 homozygous mice. The reduction ratio of neural density reached a maximum at PO 3d, decreased at PO 10d, and later showed a plateau. In a rescue experiment, an administration of NT-4/5 showed an acceleration of nerve regeneration in the homozygous mice. These findings indicate that the nt-4/5-depletion causes a delay in the regeneration of the periodontal Ruffini endings, but the delay is shortened by an exogenous administration of NT-4/5. Combined with our previous findings of bdnf-deficient mice (Harada et al. [2003] Arch Histol Cytol 66:183-194), these morphological and numerical data suggest that multiple neurotrophins such as NT-4/5 and brain-derived neurotrophic factor (BDNF) play roles in their regeneration in a stage-specific manner.

Immunohistochemistry

Denervation - methods

Trigeminal Nerve Injuries

Nerve Regeneration - physiology

Cranial Nerve Injuries - enzymology

Nerve Growth Factors - metabolism

Periodontal Ligament - metabolism

Neuroprotective Agents - metabolism

Mice, Knockout

Periodontal Ligament - cytology

Animals

Nerve Growth Factors - genetics

Mice

Mandibular Nerve - metabolism

Mechanoreceptors - metabolism

Periodontal Ligament - innervation

Details

Title: Subtitle: Involvement of neurotrophin-4/5 in regeneration of the periodontal Ruffini endings at the early stage
Creators: Shahiqul Jabbar - Division of Oral Anatomy, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan
Fumiko Harada
Megumi Aita
Megumi Ohishi
Isao Saito
Yoshiro Kawano
Akiko Suzuki
Kayoko Nozawa-Inoue
Takeyasu Maeda
Resource Type: Journal article
Publication Details: Journal of comparative neurology (1911), Vol.501(3), pp.400-412
Publisher: United States
DOI: 10.1002/cne.21256
PMID: 17245704
ISSN: 0021-9967
eISSN: 1096-9861
Language: English
Date published: 03/20/2007
Academic Unit: Periodontics
Record Identifier: 9984065717002771

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