Journal article
Involvement of transcription termination factor 2 in mitotic repression of transcription elongation
Molecular cell, Vol.14(3), pp.375-386
05/07/2004
DOI: 10.1016/S1097-2765(04)00234-5
PMID: 15125840
Abstract
All nuclear transcription is interrupted during mitosis. We examined the role of human TTF2, an RNA polymerase (Pol) I and II termination factor, in mitotic repression of transcription elongation. We find that TTF2 levels rise in the cytoplasm in S and G2 and at the onset of mitosis TTF2 translocates into the nucleus. Consistent with a role in termination of all transcription, TTF2 is the only ATP-dependent termination activity associated with Pol II transcription elongation complexes, is largely unaffected by template position, and is impervious to the phosphorylation state of the polymerase. Cells in which TTF2 levels are knocked down showed dramatic retention of Ser2 phosphorylated Pol II on mitotic chromosomes and an increase in chromosome segregation defects.
Details
- Title: Subtitle
- Involvement of transcription termination factor 2 in mitotic repression of transcription elongation
- Creators
- Yan Jiang - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USAMingyi LiuCharlotte A SpencerDavid H Price
- Resource Type
- Journal article
- Publication Details
- Molecular cell, Vol.14(3), pp.375-386
- Publisher
- United States
- DOI
- 10.1016/S1097-2765(04)00234-5
- PMID
- 15125840
- ISSN
- 1097-2765
- eISSN
- 1097-4164
- Grant note
- GM35500 / NIGMS NIH HHS
- Language
- English
- Date published
- 05/07/2004
- Academic Unit
- Biochemistry and Molecular Biology
- Record Identifier
- 9984024409102771
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