Journal article
Ion transport by primary cultures of canine tracheal epithelium: Methodology, morphology, and electrophysiology
The Journal of membrane biology, Vol.88(2), pp.149-163
06/1985
DOI: 10.1007/BF01868429
PMID: 4093958
Abstract
Canine tracheal epithelial cells were isolated by enzymatic and mechanical dispersion and cultured on permeable supports. The cells formed confluent monolayers and retained most of the morphologic characteristics of the intact epithelium, including apical microvilli, apical tight junctions, and a moderately interdigitated lateral intercellular space. The cells also retained the functional properties of the epithelium. The monolayer responded to addition of isoproterenol with the characteristic changes in cellular electrical properties expected for stimulation of Cl secretion: isoproterenol increased transepithelial voltage, depolarized apical membrane voltage, and decreased both transepithelial resistance and the ratio of apical-to-basolateral membrane resistance. Examination of the cellular response to ion substitutions and inhibitors of Cl secretion indicate that the cultured monolayers retain the same cellular mechanisms of ion transport as the intact epithelium. Thus, primary cultures of tracheal epithelium may provide a useful preparation for future studies of the mechanism and regulation of Cl secretion by airway epithelia.
Details
- Title: Subtitle
- Ion transport by primary cultures of canine tracheal epithelium: Methodology, morphology, and electrophysiology
- Creators
- Michael J Welsh
- Resource Type
- Journal article
- Publication Details
- The Journal of membrane biology, Vol.88(2), pp.149-163
- DOI
- 10.1007/BF01868429
- PMID
- 4093958
- ISSN
- 0022-2631
- eISSN
- 1432-1424
- Language
- English
- Date published
- 06/1985
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Neurosurgery; Internal Medicine
- Record Identifier
- 9984020996602771
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