Journal article
Iron-catalyzed reactions cause lipid peroxidation in the intact heart
Journal of molecular and cellular cardiology, Vol.24(9), pp.1031-1038
09/01/1992
DOI: 10.1016/0022-2828(92)91869-7
PMID: 1433319
Abstract
The chemical targets and mechanisms of iron-catalyzed oxidative injury in myocardium are poorly understood. Oxygen metabolites, in the presence of iron, can initiate free-radical chain reactions in unsaturated membrane lipids, generating lipid peroxides and causing membrane injury. We examined whether exposure to iron-catalyzed oxidative injury would increase myocardial lipid peroxide levels as injury evolved in the intact heart. Isolated, buffer perfused rabbit hearts were exposed for 30 min to 100 u
m Fe
2+/500 u
m ADP and 10 u
m H
2O
2 (IRON group,
n=5), saline vehicle (CON group,
n=6) or 500 u
m ADP and 10 u
m H
2O
2 without iron (ADP,
n=5). Lipid peroxides were measured in cytosol and membrane fractions by a new method, using the lipid peroxide-induced oxidation of exogenous GSH to GSSG, catalyzed by the enzyme glutathione peroxidase. The results indicated that iron-catalyzed lipid peroxidation occurs in the intact heart during chemically-mediated oxidative injury.
Details
- Title: Subtitle
- Iron-catalyzed reactions cause lipid peroxidation in the intact heart
- Creators
- Edward J. Lesnefsky - Case Western Reserve UniversityKenneth G.D. Allen - Colorado State UniversityFrank P. Carrea - University of Colorado HealthLawrence D. Horwitz - Colorado State University
- Resource Type
- Journal article
- Publication Details
- Journal of molecular and cellular cardiology, Vol.24(9), pp.1031-1038
- Publisher
- Elsevier Ltd
- DOI
- 10.1016/0022-2828(92)91869-7
- PMID
- 1433319
- ISSN
- 0022-2828
- eISSN
- 1095-8584
- Language
- English
- Date published
- 09/01/1992
- Academic Unit
- Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984656537602771
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