Iron oxide nanoparticles induce Pseudomonas aeruginosa growth, induce biofilm formation, and inhibit antimicrobial peptide function

Jennifer Borcherding; Jonas Baltrusaitis; Haihan Chen; Larissa Stebounova; Chia-Ming Wu; Gayan Rubasinghege; Imali A Mudunkotuwa; Juan Carlos Caraballo; Joseph Zabner; Vicki H Grassian; Alejandro P Comellas

doi:10.1039/c3en00029j

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Iron oxide nanoparticles induce Pseudomonas aeruginosa growth, induce biofilm formation, and inhibit antimicrobial peptide function

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Iron oxide nanoparticles induce Pseudomonas aeruginosa growth, induce biofilm formation, and inhibit antimicrobial peptide function

Jennifer Borcherding, Jonas Baltrusaitis, Haihan Chen, Larissa Stebounova, Chia-Ming Wu, Gayan Rubasinghege, Imali A Mudunkotuwa, Juan Carlos Caraballo, Joseph Zabner, Vicki H Grassian, …

Environmental science. Nano, Vol.1(2), pp.123-132

04/2014

DOI: 10.1039/c3en00029j

PMCID: PMC4158920

PMID: 25221673

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Abstract

Given the increased use of iron-containing nanoparticles in a number of applications, it is important to understand any effects that iron-containing nanoparticles can have on the environment and human health. Since iron concentrations are extremely low in body fluids, there is potential that iron-containing nanoparticles may influence the ability of bacteria to scavenge iron for growth, affect virulence and inhibit antimicrobial peptide (AMP) function. In this study, (PA01) and AMPs were exposed to iron oxide nanoparticles, hematite (α-Fe O ), of different sizes ranging from 2 to 540 nm (2 ± 1, 43 ± 6, 85 ± 25 and 540 ± 90 nm) in diameter. Here we show that the greatest effect on bacterial growth, biofilm formation, and AMP function impairment is found when exposed to the smallest particles. These results are attributed in large part to enhanced dissolution observed for the smallest particles and an increase in the amount of bioavailable iron. Furthermore, AMP function can be additionally impaired by adsorption onto nanoparticle surfaces. In particular, lysozyme readily adsorbs onto the nanoparticle surface which can lead to loss of peptide activity. Thus, this current study shows that co-exposure of nanoparticles and known pathogens can impact host innate immunity. Therefore, it is important that future studies be designed to further understand these types of impacts.

Details

Title: Subtitle: Iron oxide nanoparticles induce Pseudomonas aeruginosa growth, induce biofilm formation, and inhibit antimicrobial peptide function
Creators: Jennifer Borcherding - Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
Jonas Baltrusaitis - Department of Chemistry, University of Iowa, Iowa City, IA 52242, USA
Haihan Chen - Department of Chemistry, University of Iowa, Iowa City, IA 52242, USA
Larissa Stebounova - Department of Chemistry, University of Iowa, Iowa City, IA 52242, USA
Chia-Ming Wu - Department of Chemistry, University of Iowa, Iowa City, IA 52242, USA
Gayan Rubasinghege - Department of Chemistry, University of Iowa, Iowa City, IA 52242, USA
Imali A Mudunkotuwa - Department of Chemistry, University of Iowa, Iowa City, IA 52242, USA
Juan Carlos Caraballo - Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
Joseph Zabner - Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
Vicki H Grassian - Department of Chemistry, University of Iowa, Iowa City, IA 52242, USA
Alejandro P Comellas - Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Environmental science. Nano, Vol.1(2), pp.123-132
DOI: 10.1039/c3en00029j
PMID: 25221673
PMCID: PMC4158920
NLM abbreviation: Environ Sci Nano
ISSN: 2051-8153
eISSN: 2051-8161
Grant note: P30 ES005605 / NIEHS NIH HHS K01 HL080966 / NHLBI NIH HHS P01 HL091842 / NHLBI NIH HHS UL1 RR024979 / NCRR NIH HHS
Language: English
Date published: 04/2014
Academic Unit: Pulmonary, Critical Care, and Occupational Medicine; ICTS; Chemistry; Chemical and Biochemical Engineering; Internal Medicine
Record Identifier: 9984094393902771

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