Journal article
Irx1 regulates dental outer enamel epithelial and lung alveolar type II epithelial differentiation
Developmental biology, Vol.429(1), pp.44-55
09/01/2017
DOI: 10.1016/j.ydbio.2017.07.011
PMCID: PMC5599132
PMID: 28746823
Abstract
The Iroquois genes (Irx) appear to regulate fundamental processes that lead to cell proliferation, differentiation, and maturation during development. In this report, the Iroquois homeobox 1 (Irx1) transcription factor was functionally disrupted using a LacZ insert and LacZ expression demonstrated stage-specific expression during embryogenesis. Irx1 is highly expressed in the brain, lung, digits, kidney, testis and developing teeth. Irx1 null mice are neonatal lethal and this lethality it due to pulmonary immaturity. Irx1-/- mice show delayed lung maturation characterized by defective surfactant protein secretion and Irx1 marks a population of SP-C expressing alveolar type II cells. Irx1 is specifically expressed in the outer enamel epithelium (OEE), stellate reticulum (SR) and stratum intermedium (SI) layers of the developing tooth. Irx1 mediates dental epithelial cell differentiation in the lower incisors resulting in delayed growth of the lower incisors. Irx1 is specifically and temporally expressed during developmental stages and we have focused on lung and dental development in this report. Irx1+ cells are unique to the development of the incisor outer enamel epithelium, patterning of Lef-1+ and Sox2+ cells as well as a new marker for lung alveolar type II cells. Mechanistically, Irx1 regulates Foxj1 and Sox9 to control cell differentiation during development.
•Iroquois homeobox 1 (Irx1) expression is stage-specific during embryogenesis.•Irx1 null mice are neonatal lethal due to respiratory failure.•Irx1 marks specific populations of lung and dental progenitor cells.•Irx1 regulates Foxj1 and Sox9 to control cell differentiation during development.
Details
- Title: Subtitle
- Irx1 regulates dental outer enamel epithelial and lung alveolar type II epithelial differentiation
- Creators
- Wenjie Yu - Department of Anatomy and Cell Biology, and the Craniofacial Anomalies Research Center, Carver College of Medicine, The University of Iowa, Iowa City, IA 52242, USAXiao Li - Department of Cellular and Molecular Medicine, University of California, San Diego, CA, USASteven Eliason - Department of Anatomy and Cell Biology, and the Craniofacial Anomalies Research Center, Carver College of Medicine, The University of Iowa, Iowa City, IA 52242, USAMiguel Romero-Bustillos - Iowa Institute for Oral Health Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USARyan J Ries - Department of Anatomy and Cell Biology, and the Craniofacial Anomalies Research Center, Carver College of Medicine, The University of Iowa, Iowa City, IA 52242, USAHuojun Cao - Iowa Institute for Oral Health Research, College of Dentistry, The University of Iowa, Iowa City, IA 52242, USABrad A Amendt - Department of Anatomy and Cell Biology, and the Craniofacial Anomalies Research Center, Carver College of Medicine, The University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Developmental biology, Vol.429(1), pp.44-55
- DOI
- 10.1016/j.ydbio.2017.07.011
- PMID
- 28746823
- PMCID
- PMC5599132
- NLM abbreviation
- Dev Biol
- ISSN
- 0012-1606
- eISSN
- 1095-564X
- Publisher
- Elsevier Inc
- Grant note
- name: University of Iowa College of Dentistry; DOI: 10.13039/100000002, name: National Institutes of Health, award: DE13941, R90 DE024296-03
- Language
- English
- Date published
- 09/01/2017
- Academic Unit
- Orthodontics; Anatomy and Cell Biology; Endodontics; Craniofacial Anomalies Research Center; Dental Research; Internal Medicine
- Record Identifier
- 9984025444102771
Metrics
24 Record Views