Is There Excess Oxidative Stress and Damage in Eyes of Patients with Retinitis Pigmentosa?

Peter A Campochiaro; Rupert W Strauss; Lili Lu; Gulnar Hafiz; Yulia Wolfson; Syed M Shah; Raafay Sophie; Tahreem A Mir; Hendrik P Scholl

doi:10.1089/ars.2015.6327

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Is There Excess Oxidative Stress and Damage in Eyes of Patients with Retinitis Pigmentosa?

Journal article

Peer reviewed

Is There Excess Oxidative Stress and Damage in Eyes of Patients with Retinitis Pigmentosa?

Peter A Campochiaro, Rupert W Strauss, Lili Lu, Gulnar Hafiz, Yulia Wolfson, Syed M Shah, Raafay Sophie, Tahreem A Mir and Hendrik P Scholl

Antioxidants & redox signaling, Vol.23(7), pp.643-648

09/01/2015

DOI: 10.1089/ars.2015.6327

PMCID: PMC4554553

PMID: 25820114

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https://www.ncbi.nlm.nih.gov/pmc/articles/4554553View

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Abstract

Retinitis pigmentosa (RP) is a group of diseases in which a mutation in one of the large variety of genes causes death of rod photoreceptors. After rods die, cone photoreceptors gradually die resulting in constriction of visual fields and eventual blindness in many patients. Studies in animal models of RP have demonstrated that oxidative damage is a major contributor to cone cell death. In this study, we extended those findings to patients with RP, because compared to control patients, those with RP showed significant reduction in the reduced to oxidized glutathione (GSH/GSSG) ratio in aqueous humor and a significant increase in aqueous protein carbonyl content. In contrast, there was no significant decrease in the serum GSH/GSSG ratio or increase in carbonyl content of serum proteins. These data indicate that patients with RP have ocular oxidative stress and damage in the absence of manifestations of systemic oxidative stress and/or damage indicating that demonstrations of oxidative damage-induced cone cell death in animal models of RP may translate to human RP. These observations lead to the hypothesis that potent antioxidants will promote cone survival and function in patients with RP and that the aqueous GSH/GSSG ratio and carbonyl content on proteins may provide useful biomarkers. Antioxid. Redox Signal. 23, 643-648.

Animals

Aqueous Humor - metabolism

Eye - metabolism

Eye - pathology

Glutathione - blood

Glutathione - metabolism

Glutathione Disulfide - metabolism

Humans

Oxidative Stress

Protein Carbonylation

Retinitis Pigmentosa - metabolism

Retinitis Pigmentosa - pathology

Details

Title: Subtitle: Is There Excess Oxidative Stress and Damage in Eyes of Patients with Retinitis Pigmentosa?
Creators: Peter A Campochiaro - Johns Hopkins University
Rupert W Strauss - Johns Hopkins University School of Medicine
Lili Lu - Johns Hopkins Medicine
Gulnar Hafiz - Johns Hopkins University
Yulia Wolfson - Johns Hopkins Medicine
Syed M Shah - Johns Hopkins Medicine
Raafay Sophie - Johns Hopkins Medicine
Tahreem A Mir - Johns Hopkins University
Hendrik P Scholl - Johns Hopkins Medicine
Resource Type: Journal article
Publication Details: Antioxidants & redox signaling, Vol.23(7), pp.643-648
DOI: 10.1089/ars.2015.6327
PMID: 25820114
PMCID: PMC4554553
NLM abbreviation: Antioxid Redox Signal
ISSN: 1523-0864
eISSN: 1557-7716
Grant note: EY05951 / NEI NIH HHS
Language: English
Date published: 09/01/2015
Academic Unit: Ophthalmology and Visual Sciences
Record Identifier: 9984755389702771

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