Is the onset of actin histidine methylation under development control in the chick embryo

K A Cass; E B Clark; P A Rubenstein

doi:10.1016/0003-9861(83)90084-x

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Is the onset of actin histidine methylation under development control in the chick embryo

Journal article

Peer reviewed

Is the onset of actin histidine methylation under development control in the chick embryo

K A Cass, E B Clark and P A Rubenstein

Archives of biochemistry and biophysics, Vol.225(2), pp.731-739

09/1983

DOI: 10.1016/0003-9861(83)90084-x

PMID: 6625608

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Abstract

It had previously been reported (B. Krzysik, J. P. Vergnes, and I. R. McManus (1971) Arch. Biochem. Biophys. 146, 34-45) that prior to day 11 of embryonic life chick skeletal muscle actin contained little or no 3-methylhistidine, and that between Day 11 and 18, the degree of actin histidine methylation increased until it leveled off at 1 mol of 3-methylhistidine/mol actin. This is the value seen in adult muscle and nonmuscle actins so far analyzed. To determine whether this delayed onset of actin methylation occurred simultaneously throughout the organism or differed from tissue to tissue, the 3-methylhistidine content of cardiac muscle actin from Day 2 of embryonic life to hatching and of brain actin at Days 9, 11, and 14 were analyzed. These results, obtained by analyzing unlabeled actin samples as well as samples labeled in vivo with [3H]histidine, showed that at all stages, 1 mol of 3-methylhistidine was present per mol of actin. When skeletal muscle samples obtained from Day 11 to 18 embryos were analyzed 1 mol of 3-methylhistidine/mol of actin was observed. Thus, in the chick embryo, contrary to those reports published earlier, it was found that actin histidine methylation is not under developmental control.

Brain - embryology

Actins - isolation & purification

Molecular Weight

Heart - embryology

Muscles - embryology

Actins - metabolism

Histidine - metabolism

Organ Specificity

Chick Embryo

Brain - metabolism

Animals

Myocardium - metabolism

Methylation

Muscles - metabolism

Details

Title: Subtitle: Is the onset of actin histidine methylation under development control in the chick embryo
Creators: K A Cass
E B Clark
P A Rubenstein
Resource Type: Journal article
Publication Details: Archives of biochemistry and biophysics, Vol.225(2), pp.731-739
Publisher: United States
DOI: 10.1016/0003-9861(83)90084-x
PMID: 6625608
ISSN: 0003-9861
eISSN: 1096-0384
Grant note: HL-07413 / NHLBI NIH HHS HL-14388 / NHLBI NIH HHS
Language: English
Date published: 09/1983
Academic Unit: Stead Family Department of Pediatrics; Biochemistry and Molecular Biology; Internal Medicine
Record Identifier: 9984024539102771

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