Journal article
Isolated Effects of Plasma Freezing versus Thawing on Metabolite Stability
Metabolites, Vol.12(11), p.1098
11/11/2022
DOI: 10.3390/metabo12111098
PMCID: PMC9693613
PMID: 36422241
Abstract
Freezing and thawing plasma samples is known to perturb metabolite stability. However, no study has systematically tested how different freezing and thawing methods affect plasma metabolite levels. The objective of this study was to isolate the effects of freezing from thawing on mouse plasma metabolite levels, by comparing a matrix of freezing and thawing conditions through 10 freeze-thaw cycles. We tested freezing with liquid nitrogen (LN2), at -80 degrees C, or at -20 degrees C, and thawing quickly in room temperature water or slowly on ice. Plasma samples were extracted and the relative abundance of 87 metabolites was obtained via liquid chromatography-mass spectrometry (LC-MS). Observed changes in metabolite abundance by treatment group correlated with the amount of time it took for samples to freeze or thaw. Thus, snap-freezing with LN2 and quick-thawing with water led to minimal changes in metabolite levels. Conversely, samples frozen at -20 degrees C exhibited the most changes in metabolite levels, likely because freezing required about 4 h, versus freezing instantaneously in LN2. Overall, our results show that plasma samples subjected to up to 10 cycles of LN2 snap-freezing with room temperature water quick-thawing exhibit remarkable metabolomic stability.
Details
- Title: Subtitle
- Isolated Effects of Plasma Freezing versus Thawing on Metabolite Stability
- Creators
- Jane L. Buchanan - University of IowaJovan Tormes Vaquerano - Univ Iowa, Dept Mol Physiol & Biophys, Carver Coll Med, Iowa City, IA 52240 USAEric B. Taylor - Univ Iowa, Dept Mol Physiol & Biophys, Carver Coll Med, Iowa City, IA 52240 USA
- Resource Type
- Journal article
- Publication Details
- Metabolites, Vol.12(11), p.1098
- DOI
- 10.3390/metabo12111098
- PMID
- 36422241
- PMCID
- PMC9693613
- NLM abbreviation
- Metabolites
- ISSN
- 2218-1989
- eISSN
- 2218-1989
- Publisher
- Mdpi
- Number of pages
- 12
- Language
- English
- Date published
- 11/11/2022
- Academic Unit
- Molecular Physiology and Biophysics; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984383904102771
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