Isolating RNA with the cationic surfactant, Catrimox-14

C E Dahle; D E Macfarlane

doi:10.1385/0-89603-494-1:19

Back

Journal article

Isolating RNA with the cationic surfactant, Catrimox-14

C E Dahle and D E Macfarlane

Methods in molecular biology (Clifton, N.J.), Vol.86, pp.19-21

1998

DOI: 10.1385/0-89603-494-1:19

PMID: 9664447

View Online

Abstract

Cationic surfactants precipitate nucleic acids, presumably by forming reverse micelles in which the ahphatic tails of the surfactant face the aqueous phase, and the cationic head groups bind to the nucleic acid electrostatically. The precipitate can be dissolved in organic solvents, or it can be redissolved in water by the addition of salt. Selected cationic surfactants are capable of lysing cells, by solubilizmg their protem and lipid components.

DNA - isolation & purification

Humans

Lithium Chloride

Molecular Biology - methods

Quaternary Ammonium Compounds

RNA - blood

RNA - isolation & purification

RNA, Bacterial - isolation & purification

RNA, Plant - isolation & purification

Surface-Active Agents

Trimethyl Ammonium Compounds

Details

Title: Subtitle: Isolating RNA with the cationic surfactant, Catrimox-14
Creators: C E Dahle - University of Iowa
D E Macfarlane - University of Iowa
Resource Type: Journal article
Publication Details: Methods in molecular biology (Clifton, N.J.), Vol.86, pp.19-21
DOI: 10.1385/0-89603-494-1:19
PMID: 9664447
ISSN: 1064-3745
eISSN: 1940-6029
Language: English
Date published: 1998
Academic Unit: Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
Record Identifier: 9984359583402771

Metrics

11 Record Views

See more details