Journal article
Isolation and Whole‐Genome Sequencing of Environmental Campylobacter
Current protocols in microbiology (Online), Vol.51(1), pp.e64-n/a
11/2018
DOI: 10.1002/cpmc.64
PMID: 30369079
Abstract
As a leading cause of bacterial‐derived gastroenteritis worldwide, Campylobacter has a significant impact on human health. In the developed world, most campylobacteriosis cases are attributed to the consumption of undercooked, contaminated poultry; however, it has been shown that Campylobacter can be transmitted to humans through contaminated water and other types of food, including beef and milk. As such, high‐resolution microbial source‐tracking is essential for health department officials to determine the source(s) of Campylobacter outbreaks. For these reasons, this protocol provides the techniques needed for isolation of Campylobacter from agricultural and environmental sources, as well as human clinical specimens. Additionally, we describe a simple method for preparing high‐quality genomic DNA that can be used for whole‐genome sequencing and downstream bioinformatics analyses of Campylobacter genotypes. © 2018 by John Wiley & Sons, Inc.
Details
- Title: Subtitle
- Isolation and Whole‐Genome Sequencing of Environmental Campylobacter
- Creators
- Brittni R. Kelley - University of Tennessee at KnoxvilleJ. Christopher Ellis - Oak Ridge National LaboratoryDoug Hyatt - University of Tennessee at KnoxvilleDan Jacobson - Oak Ridge National LaboratoryJeremiah Johnson - University of Tennessee at Knoxville
- Resource Type
- Journal article
- Publication Details
- Current protocols in microbiology (Online), Vol.51(1), pp.e64-n/a
- DOI
- 10.1002/cpmc.64
- PMID
- 30369079
- NLM abbreviation
- Curr Protoc Microbiol
- ISSN
- 1934-8525
- eISSN
- 1934-8533
- Number of pages
- 16
- Grant note
- Center for Bioenergy Innovation Department of Energy, Laboratory Directed Research and Development (ProjectID 8321) U.S. Department of Energy Bioenergy Research Center Office of Science of the U.S. Department of Energy (DE‐AC05‐00OR22725) U.S. Department of Energy Office of Science Oak Ridge National Laboratory Office of Biological and Environmental Research in the DOE Office of Science Office of Biological and Environmental Research Plant‐Microbe Interfaces Scientific Focus Area in the Genomic Science Program Joint Directed Research and Development (R013318090) University of Tennessee
- Language
- English
- Date published
- 11/2018
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984696725502771
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