Journal article
Isolation and culture of cells derived from human cerebral microvessels
Cell and tissue research, Vol.249(3), pp.657-667
09/1987
DOI: 10.1007/BF00217338
PMID: 3664609
Abstract
Microvessels were isolated from non-neoplastic human cerebral cortical fragments resected for treatment of intractable seizure disorder. The microvessels were incubated in modified Lewis medium with 20 or 30% fetal bovine serum. Within 1-2 weeks, two cell populations emerged from the isolates. One type of cells had polygonal morphology, showed density-dependent contact inhibition at confluence in vitro, showed lectin-binding characteristics of endothelium (but only moderate positivity for factor VIII antigen), demonstrated induction of gamma-glutamyl transpeptidase when exposed to astrocyte-conditioned media, and responded to insulin by a pronounced increase in DNA synthesis. The other variety of cells grew in vitro more slowly in irregular strands separated by clear zones, showed ultrastructural features of smooth muscle, and isoelectric focusing of cell proteins revealed the presence of smooth-muscle-specific alpha-isoactin. Both types of cells could be serially subcultured. The ability to isolate and grow the two cell types, tentatively identified as human cerebral microvascular endothelium and smooth muscle, may facilitate studies of human blood-brain barrier function as well as the pathogenesis of cerebral microangiopathies unique to the human brain.
Details
- Title: Subtitle
- Isolation and culture of cells derived from human cerebral microvessels
- Creators
- HarryV VintersSusan ReavePenny CostelloJohnP GirvinStevenA Moore
- Resource Type
- Journal article
- Publication Details
- Cell and tissue research, Vol.249(3), pp.657-667
- DOI
- 10.1007/BF00217338
- PMID
- 3664609
- ISSN
- 0302-766X
- eISSN
- 1432-0878
- Language
- English
- Date published
- 09/1987
- Academic Unit
- Pathology
- Record Identifier
- 9984047713702771
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