Isolation, characterization, and localization of the inositol 1,4,5-trisphosphate receptor protein in Xenopus laevis oocytes

Jan B Parys; Suzanne W Sernett; Sylvain Delisle; Peter M Snyder; Michael J Welsh; Kevin P Campbell

doi:10.1016/S0021-9258(19)37028-0

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Isolation, characterization, and localization of the inositol 1,4,5-trisphosphate receptor protein in Xenopus laevis oocytes

Journal article

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Peer reviewed

Isolation, characterization, and localization of the inositol 1,4,5-trisphosphate receptor protein in Xenopus laevis oocytes

Jan B Parys, Suzanne W Sernett, Sylvain Delisle, Peter M Snyder, Michael J Welsh and Kevin P Campbell

The Journal of biological chemistry, Vol.267(26), pp.18776-18782

1992

DOI: 10.1016/S0021-9258(19)37028-0

PMID: 1326534

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Abstract

Inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) induces Ca2+ oscillations and waves in Xenopus laevis oocytes. Microsomes from oocytes exhibit high-affinity binding for Ins(1,4,5)P3, and demonstrate Ins(1,4,5)P3-induced Ca2+ release. The Ins(1,4,5)P3 receptor (InsP3R) was purified from oocyte microsomes as a large tetrameric complex and shown to have a monomer molecular mass of 256 kDa, compared with 273 kDa for the brain InsP3R. Binding to the oocyte receptor is highly specific for Ins(1,4,5)P3 and is inhibited by heparin (IC50, 2 micrograms/ml). Immunoblot analysis revealed that an antibody against the C-terminal sequence of the brain receptor recognized the oocyte receptor. These results, in addition to the difference in pattern obtained after limited proteolysis, suggest that the oocyte InsP3R is a new shorter isoform of the mammalian brain type I InsP3R. Immunofluorescence experiments indicated the presence of the InsP3R in the cortical layer and the perinuclear endoplasmic reticulum of the oocyte. However, immunological and biochemical experiments did not reveal the presence of the ryanodine receptor. The presence of an InsP3R and the absence of a ryanodine receptor support the importance of Ins(1,4,5)P3 in Ca2+ handling by oocytes and particularly in the induction of Ca2+ oscillations and waves.

Fundamental and applied biological sciences. Psychology

Biological and medical sciences

Cell receptors

Miscellaneous

Molecular and cellular biology

Cell structures and functions

Details

Title: Subtitle: Isolation, characterization, and localization of the inositol 1,4,5-trisphosphate receptor protein in Xenopus laevis oocytes
Creators: Jan B Parys - Univ. Iowa coll. medicine, Howard Hughes medical inst., Iowa City IA 52242, United States
Suzanne W Sernett - Univ. Iowa coll. medicine, Howard Hughes medical inst., Iowa City IA 52242, United States
Sylvain Delisle - Univ. Iowa coll. medicine, Howard Hughes medical inst., Iowa City IA 52242, United States
Peter M Snyder - Univ. Iowa coll. medicine, Howard Hughes medical inst., Iowa City IA 52242, United States
Michael J Welsh - Univ. Iowa coll. medicine, Howard Hughes medical inst., Iowa City IA 52242, United States
Kevin P Campbell - Univ. Iowa coll. medicine, Howard Hughes medical inst., Iowa City IA 52242, United States
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.267(26), pp.18776-18782
DOI: 10.1016/S0021-9258(19)37028-0
PMID: 1326534
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: American Society for Biochemistry and Molecular Biology; Bethesda, MD
Language: English
Date published: 1992
Academic Unit: Neurology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Iowa Neuroscience Institute; Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Neurosurgery; Medicine Administration; Internal Medicine
Record Identifier: 9984020728402771

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