Journal article
Isolation, characterization and targeted disruption of mouse Ppia : Cyclophilin A is not essential for mammalian cell viability
Genomics (San Diego, Calif.), Vol.68(2), pp.167-178
2000
DOI: 10.1006/geno.2000.6295
PMID: 10964515
Abstract
Cyclophilins (CyPs) are a family of proteins found in organisms ranging from prokaryotes to humans. These molecules exhibit peptidyl-prolyl isomerase activity in vitro, suggesting that they influence the conformation of proteins in cells. CyPs also bind with varying affinities to the immunosuppressive drug cyclosporin A (CsA), a compound used clinically to prevent allograft rejection. The founding member of the family, cyclophilin A (CyPA), is an abundant, ubiquitously expressed protein of unknown function that binds with nanomolar affinity to CsA. Here, we describe the isolation and characterization of mouse Ppia (mPpia), the gene encoding CyPA. Ppia was isolated using a PCR screen that distinguishes the expressed gene from multiple pseudogenes present in the mouse genome. mPpia consists of 5 exons and 4 introns spanning roughly 4.5 kb and maps to chromosome 11 near the centromere. Sequence analysis of a 369-bp fragment from the proximal promoter region of mPpia revealed the presence of a TATA box and sites recognized by several transcriptional regulators, including Sp1, AP-2, GATA factors, c-Myb, and NF-IL-6. This region is sufficient to drive high-level reporter gene expression in transfected cells. Both copies of Ppia were disrupted in murine embryonic stem (ES) cells via gene targeting. Ppia(-/-) ES cells grow normally and differentiate into hematopoeitic precursor cells in vitro, indicating that CyPA is not essential for mammalian cell viability.
Details
- Title: Subtitle
- Isolation, characterization and targeted disruption of mouse Ppia : Cyclophilin A is not essential for mammalian cell viability
- Creators
- John COLGAN - Department of Microbiology and Department of Medicine, Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, New York 10032, United StatesMohammed ASMAL - Department of Microbiology and Department of Medicine, Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, New York 10032, United StatesJeremy LUBAN - Department of Microbiology and Department of Medicine, Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, New York 10032, United States
- Resource Type
- Journal article
- Publication Details
- Genomics (San Diego, Calif.), Vol.68(2), pp.167-178
- DOI
- 10.1006/geno.2000.6295
- PMID
- 10964515
- NLM abbreviation
- Genomics
- ISSN
- 0888-7543
- eISSN
- 1089-8646
- Publisher
- Elsevier; San Diego, CA
- Language
- English
- Date published
- 2000
- Academic Unit
- Anatomy and Cell Biology; Immunology; Internal Medicine
- Record Identifier
- 9984025333502771
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