Journal article
Isolation of TSH and LH/CG Receptor cDNAs from Human Thyroid: Regulation by Tissue Specific Splicing
Molecular endocrinology (Baltimore, Md.), Vol.4(8), pp.1264-1276
08/1990
DOI: 10.1210/mend-4-8-1264
PMID: 2293030
Abstract
A TSH receptor (TSH-R) cDNA has been isolated from a human thyroid lambda GT11 library. Unexpectedly, several cDNAs encoding the human LH/CG receptor (LH/CG-R), previously thought to be expressed solely in gonadal cells, were also isolated from the thyroid library. The receptors are structurally related, consisting of a signal sequence, a large extracellular amino terminal domain, seven membrane spanning domains, and a short carboxyl-terminal portion. The TSH-R is encoded by a single 4.2 kilobase mRNA specific to the thyroid. Introns were not present in any hTSH-R cDNAs examined, however, sequencing of several LH/CG-R cDNAs and RNase protection experiments demonstrated that the majority of hLH/CG-R mRNA in the thyroid is incompletely spliced. Consequently, tissue-specific splicing may be an important step in the regulation of the glycoprotein hormone receptor family.
Details
- Title: Subtitle
- Isolation of TSH and LH/CG Receptor cDNAs from Human Thyroid: Regulation by Tissue Specific Splicing
- Creators
- A. Lindsay FrazierLinda S RobbinsPhilip J StorkRolf SprengelDeborah L SegaloffRoger D Cone
- Resource Type
- Journal article
- Publication Details
- Molecular endocrinology (Baltimore, Md.), Vol.4(8), pp.1264-1276
- DOI
- 10.1210/mend-4-8-1264
- PMID
- 2293030
- NLM abbreviation
- Mol Endocrinol
- ISSN
- 0888-8809
- eISSN
- 1944-9917
- Language
- English
- Date published
- 08/1990
- Academic Unit
- Molecular Physiology and Biophysics; Obstetrics and Gynecology
- Record Identifier
- 9984083836002771
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