Journal article
Isolation of YAC clones from the pericentromeric region of chromosome 10 and development of new genetic markers linked to the multiple endocrine neoplasia type 2A gene
Henry Ford Hospital medical journal, Vol.40(3-4), pp.210-214
01/01/1992
PMID: 1362407
Abstract
Genetic linkage mapping and contig assembly using yeast artificial chromosome (YAC) technology form the basis of our strategy to clone and define the genomic structure of the pericentromeric region of chromosome 10 containing the multiple endocrine neoplasia type 2A gene. Thus far YAC walks have been initiated from five chromosome 10 pericentromeric loci including RBP3, D10S94, RET, D10Z1, and FNRB. Long range pulsed-field gel electrophoresis maps are constructed from the YACs isolated to define clone overlaps and to identify putative CpG islands. Bidirectional YAC walks are continued by rescreening the YAC library with sequence-tagged site assays developed from end-clones. Several new restriction fragment length polymorphisms and simple sequence repeat polymorphism markers have been identified from the YAC clones. In particular, two highly informative (CA)n dinucleotide repeat markers, sTCL-1 from proximal chromosome 10p (16 alleles, PIC = 0.68) and sJRH-1 from the RBP3 locus (18 alleles, PIC = 0.88), provide useful reagents for a polymerase chain reaction-based predictive genetic test that can be performed rapidly from small amounts of DNA.
Details
- Title: Subtitle
- Isolation of YAC clones from the pericentromeric region of chromosome 10 and development of new genetic markers linked to the multiple endocrine neoplasia type 2A gene
- Creators
- T C LairmoreJ R HoweS DouR VeileJ A Korte-SarfatyS A WellsH Donis-Keller
- Resource Type
- Journal article
- Publication Details
- Henry Ford Hospital medical journal, Vol.40(3-4), pp.210-214
- PMID
- 1362407
- ISSN
- 0018-0416
- Language
- English
- Date published
- 01/01/1992
- Academic Unit
- Surgery
- Record Identifier
- 9984322926102771
Metrics
8 Record Views