Journal article
Isolation of neurons suitable for patch-clamping from adult mammalian central nervous systems
Journal of neuroscience methods, Vol.16(3), pp.227-238
1986
DOI: 10.1016/0165-0270(86)90040-3
PMID: 3523050
Abstract
A method is described for the isolation of neurons from defined regions of the mammalian central nervous system, by a combination of mechanical and enzymatic means. The procedure liberates neurons free of cellular debris and glial investments, allowing the formation of giga-ohm seals with patch clamp electrodes. The characteristic morphology of neurons is maintained, together with the diversity of active channels evident in the intact nervous system.
Details
- Title: Subtitle
- Isolation of neurons suitable for patch-clamping from adult mammalian central nervous systems
- Creators
- Alan R KayRobert K.S Wong
- Resource Type
- Journal article
- Publication Details
- Journal of neuroscience methods, Vol.16(3), pp.227-238
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0165-0270(86)90040-3
- PMID
- 3523050
- ISSN
- 0165-0270
- eISSN
- 1872-678X
- Language
- English
- Date published
- 1986
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9983992097102771
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