Journal article
Isolation of replication-competent molecular clones of visna virus
Virology (New York, N.Y.), Vol.181(1), pp.228-240
1991
DOI: 10.1016/0042-6822(91)90488-W
PMID: 1847257
Abstract
Visna virus is the prototypic member of a subfamily of retroviruses responsible for slow infections of animals and humans. As a part of our investigation of the functions of viral gene products in virus replication, we have isolated three infectious molecular clones and determined the complete nucleotide sequences of two of the clones. We have also characterized the progeny of the biologically cloned viral stocks and of the infectious clones and document considerable heterogeneity in plaque size and antigenic phenotype of the former that is reduced to near homogeneity in the progeny of the infectious clones. It thus should now be possible to trace the emergence of antigenic variants of visna virus as well as ascribe defined functions to structural and regulatory genes of the virus in determining neurovirulence and the slow tempo of infection.
Details
- Title: Subtitle
- Isolation of replication-competent molecular clones of visna virus
- Creators
- Katherine A Staskus - Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, USAErnest F Retzel - Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, USAElizabeth D Lewis - Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, USAJanet L Silsby - Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, USAS.T Sheila Cyr - Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, USAJeffrey M Rank - Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, USASteven W Wietgrefe - Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, USAAshley T Haase - Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, USARonald Cook - Recombinant DNA Lab, Department of Microbiology, University of Minnesota, Milligen-Biosearch, Inc., Novato, California, USADavid Fast - Recombinant DNA Lab, Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, USAPaul T Geiser - Recombinant DNA Lab, Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, USAJohn T Harty - Recombinant DNA Lab, Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, USASelene H Kong - Recombinant DNA Lab, Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, USACarol J Lahti - Recombinant DNA Lab, Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, USAThomas P Neufeld - Recombinant DNA Lab, Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, USAThomas E Porter - Recombinant DNA Lab, Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, USAElizabeth Shoop - Recombinant DNA Lab, Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, USAKaren R Zachow - Recombinant DNA Lab, Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, USA
- Resource Type
- Journal article
- Publication Details
- Virology (New York, N.Y.), Vol.181(1), pp.228-240
- DOI
- 10.1016/0042-6822(91)90488-W
- PMID
- 1847257
- NLM abbreviation
- Virology
- ISSN
- 0042-6822
- eISSN
- 1096-0341
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 1991
- Academic Unit
- Pathology
- Record Identifier
- 9984047895302771
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