Journal article
Isolation of virally-infected insect cells from a population containing infected and uninfected cells
Biotechnology techniques, Vol.9(12), pp.897-900
12/1995
DOI: 10.1007/BF00158543
Abstract
A method was developed that can be used to isolate virally-infected insect cells from a mixed population containing infected and uninfected cells. Specifically, Spodoptera frugiperda Sf-9 cells infected with the Autographa californica multiple nuclear polyhedrosis virus were treated with a primary antibody specific for the gp64 protein present on the surface of virally-infected cells and a secondary antibody labeled with a fluorochrome. The resulting labeled cells were isolated by using fluorescence-activated cell sorting.
Details
- Title: Subtitle
- Isolation of virally-infected insect cells from a population containing infected and uninfected cells
- Creators
- Alexander MatschinerJonathan S DordickDavid W Murhammer
- Resource Type
- Journal article
- Publication Details
- Biotechnology techniques, Vol.9(12), pp.897-900
- DOI
- 10.1007/BF00158543
- ISSN
- 0951-208X
- eISSN
- 1573-6784
- Language
- English
- Date published
- 12/1995
- Academic Unit
- Chemical and Biochemical Engineering
- Record Identifier
- 9984003933602771
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