Journal article
JAK2 associates with the erythropoietin receptor and is tyrosine phosphorylated and activated following stimulation with erythropoietin
Cell (Cambridge), Vol.74(2), pp.227-236
1993
DOI: 10.1016/0092-8674(93)90414-L
PMID: 8343951
Abstract
Erythropoietin (EPO) regulates the proliferation and differentiation of erythroid cells through interaction with its receptor (EPOR). Although EPOR is a member of the cytokine receptor superfamily and lacks a kinase domain, EPO induces tyrosine phosphorylation, which is correlated with gene transcription and mitogenesis. Here we demonstrate that EPO induces tyrosine phosphorylation of JAK2 kinase and activates its in vitro autophosphorylation. Using EPOR mutants, phosphorylation and activation of kinase activity correlate with the induction of mitogenesis. Furthermore, JAK2 physically associates with a membrane-proximal region of the EPOR cytoplasmic domain that is required for biological activity. The results support the hypothesis that JAK2 is the kinase that couples EPO binding to tyrosine phosphorylation and mitogenesis.
Details
- Title: Subtitle
- JAK2 associates with the erythropoietin receptor and is tyrosine phosphorylated and activated following stimulation with erythropoietin
- Creators
- Bruce A Witthuhn - Department of Biochemistry St. Jude Children's Research Hospital Memphis, Tennessee 38105 USAFrederick W Quelle - Department of Biochemistry St. Jude Children's Research Hospital Memphis, Tennessee 38105 USAOlli Silvennoinen - Department of Pharmacology New York University Medical Center New York, New York 10016 USATaolin Yi - Department of Biochemistry St. Jude Children's Research Hospital Memphis, Tennessee 38105 USABo Tang - Department of Biochemistry St. Jude Children's Research Hospital Memphis, Tennessee 38105 USAOsamu Miura - First Department of Medicine Toyko Medical and Dental University Tokyo JapanJames N Ihle - Department of Biochemistry St. Jude Children's Research Hospital Memphis, Tennessee 38105 USA
- Resource Type
- Journal article
- Publication Details
- Cell (Cambridge), Vol.74(2), pp.227-236
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0092-8674(93)90414-L
- PMID
- 8343951
- ISSN
- 0092-8674
- eISSN
- 1097-4172
- Language
- English
- Date published
- 1993
- Academic Unit
- Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984040246902771
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