Journal article
JG cell expression and partial regulation of a human renin genomic transgene driven by a minimal renin promoter
American journal of physiology. Renal physiology, Vol.277(4), pp.F634-F642
10/01/1999
DOI: 10.1152/ajprenal.1999.277.4.F634
PMID: 10516288
Abstract
In the kidney, renin gene expression is exquisitely localized to the juxtaglomerular (JG) cells lining the afferent arteriole, having the capacity to regulate renin synthesis in response to a variety of physiological cues. We investigated human renin gene expression in transgenic mice containing a genomic construct driven by 149 bp of its proximal promoter to elucidate whether this was sufficient to confer JG-specific expression. Whereas human renin mRNA was permissively expressed in most tissues, the transgene was expressed mainly in JG cells in the kidney. Active human renin and human prorenin were found in the systemic circulation at levels consistent with previous transgenic models. Remarkably, two lines displayed an appropriate upregulation of transgene mRNA in response to angiotensin-converting enzyme inhibition, and two lines exhibited a downregulation of transgene mRNA in response to subpressor and pressor doses of ANG II. Our results suggest that 149 bp of the human renin proximal promoter, in a context of a genomic construct, are sufficient to confer human renin expression in renal JG cells and at least some aspects of appropriate regulation.
Details
- Title: Subtitle
- JG cell expression and partial regulation of a human renin genomic transgene driven by a minimal renin promoter
- Creators
- Patrick L Sinn - Departments of Internal Medicine and Physiology and Biophysics, The University of Iowa College of Medicine, Iowa City, Iowa 52242Xiaoji Zhang - Departments of Internal Medicine and Physiology and Biophysics, The University of Iowa College of Medicine, Iowa City, Iowa 52242Curt D Sigmund - Departments of Internal Medicine and Physiology and Biophysics, The University of Iowa College of Medicine, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Renal physiology, Vol.277(4), pp.F634-F642
- DOI
- 10.1152/ajprenal.1999.277.4.F634
- PMID
- 10516288
- ISSN
- 1931-857X
- eISSN
- 1522-1466
- Language
- English
- Date published
- 10/01/1999
- Academic Unit
- Molecular Physiology and Biophysics; Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Neuroscience and Pharmacology
- Record Identifier
- 9984093466502771
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