Journal article
Jarid2 Is Among a Set of Genes Differentially Regulated by Nkx2.5 During Outflow Tract Morphogenesis
Developmental dynamics, Vol.239(7), pp.2024-2033
07/2010
DOI: 10.1002/dvdy.22341
PMCID: PMC2903008
PMID: 20549724
Abstract
Nkx2.5, a transcription factor implicated in human congenital heart disease, is required for regulation of second heart field (SHF) progenitors contributing to outflow tract (OFT). Here, we define a set of genes (Lrrn1, Elovl2, Safb, Slc39a6, Khdrbs1, Hoxb4, Fez1, Ccdc117, Jarid2, Nrcam, and Enpp3) expressed in SHF containing pharyngeal arch tissue whose regulation is dependent on Nkx2.5. Further investigation shows that Jarid2, which has been implicated in OFT morphogenesis, is a direct target of Nkx2.5 regulation. Jarid2 expression expression was up-regulated in SHF mesoderm of Nkx2.5-deficient embryos. Chromatin immunoprecipitation analysis showed Nkx2.5 interaction with consensus binding sites in the Jarid2 promoter in pharyngeal arch cells. Finally, Jarid2 promoter activity and mRNA expression levels were down. regulated by Nkx2.5 overexpression. Given the role of Jarid2 as a regulator of early cardiac proliferation, these findings highlight Jarid2 as one of several potential mediators of the critical role played by Nkx2.5 during OFT morphogenesis. Developmental Dynamics 239:2024-2033, 2010. (C) 2010 Wiley-Liss, Inc.
Details
- Title: Subtitle
- Jarid2 Is Among a Set of Genes Differentially Regulated by Nkx2.5 During Outflow Tract Morphogenesis
- Creators
- Jeremy L. Barth - Medical University of South CarolinaChristopher D. Clark - Medical University of South CarolinaVictor M. Fresco - Medical University of South CarolinaEllen P. Knoll - Medical University of South CarolinaBenjamin Lee - Medical University of South CarolinaW. Scott Argraves - Medical University of South CarolinaKyu-Ho Lee - Medical University of South Carolina
- Resource Type
- Journal article
- Publication Details
- Developmental dynamics, Vol.239(7), pp.2024-2033
- Publisher
- Wiley
- DOI
- 10.1002/dvdy.22341
- PMID
- 20549724
- PMCID
- PMC2903008
- ISSN
- 1058-8388
- eISSN
- 1097-0177
- Number of pages
- 10
- Grant note
- UL1RR029882 / NATIONAL CENTER FOR RESEARCH RESOURCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Research Resources (NCRR) P20 RR015434 / NCRR; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Research Resources (NCRR) P20 RR016434; HL095067 / NIH NCRR; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Research Resources (NCRR) NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01HL095067 / NATIONAL HEART, LUNG, AND BLOOD INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI)
- Language
- English
- Date published
- 07/2010
- Academic Unit
- Obstetrics and Gynecology
- Record Identifier
- 9984697043102771
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