Journal article
K +- and temperature-evoked taurine efflux from hypothalamic astrocytes
Neuroscience letters, Vol.119(1), pp.23-26
1990
DOI: 10.1016/0304-3940(90)90746-V
PMID: 2097579
Abstract
Hypothalamic astrocytes in culture released taurine, a suspected inhibitory amino acid neurotransmitter/neuromodulator/osmoregulator, in response to isoosmotically increasing extracellular K
+ in a dose-dependent fashion. In the absence of added Ca
2+, basal release levels rose to approach those obtained after exposure to 60 mM K
+ in the presence of 2.5 mM Ca
2+, and were only partially lowered by the addition of 10 mM Mg
2+. Stimulation with K
+ (60 mM) did not further increase taurine efflux above the high basal levels seen in the absence of Ca
2+. Under standard conditions complete replacement of Na
+ with choline Cl had little effect on basal taurine release, but reduced K
+-evoked (60 mM) efflux by 60%. The temperature dependence of the basal levels of taurine released from hypothalamic astrocytes was similar to that seen for cultured cerebellar astrocytes and neurons over the range 5–50°C. Taurine release increased from 5 to 15°C, remained constant between 15 and 33°C, decreased between 33 and 37°C and increased thereafter. The infection point of increased basal taurine release seen around 37°C (most prominent in astrocytes), may be of physiological significance. Results presented also show that the ion (Na
+, Ca
2+ and K
+) sensitivities of taurine efflux for cultured hypothalamic astrocytes are similar to those previously reported for cultured astrocytes from the cerebellum.
Details
- Title: Subtitle
- K +- and temperature-evoked taurine efflux from hypothalamic astrocytes
- Creators
- Gary A Tigges - Department of Pharmacology, College of Medicine, University of Iowa, Iowa City, IA 52242 U.S.ARobert A Philibert - Department of Pharmacology, College of Medicine, University of Iowa, Iowa City, IA 52242 U.S.AGary R Dutton - Department of Pharmacology, College of Medicine, University of Iowa, Iowa City, IA 52242 U.S.A
- Resource Type
- Journal article
- Publication Details
- Neuroscience letters, Vol.119(1), pp.23-26
- DOI
- 10.1016/0304-3940(90)90746-V
- PMID
- 2097579
- NLM abbreviation
- Neurosci Lett
- ISSN
- 0304-3940
- eISSN
- 1872-7972
- Publisher
- Elsevier Ireland Ltd
- Language
- English
- Date published
- 1990
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Psychiatry; Iowa Neuroscience Institute; Neuroscience and Pharmacology
- Record Identifier
- 9984003939502771
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