Journal article
K+ channel reorganization and homeostatic plasticity during postembryonic development: biophysical and genetic analyses in acutely dissociated Drosophila central neurons
Journal of neurogenetics, Vol.30(3-4), pp.259-275
10/01/2016
DOI: 10.1080/01677063.2016.1255212
PMCID: PMC5918286
PMID: 27868467
Abstract
Intrinsic electric activities of neurons play important roles in establishing and refining neural circuits during development. However, how the underlying ionic currents undergo postembryonic reorganizations remains largely unknown. Using acutely dissociated neurons from larval, pupal, and adult Drosophila brains, we show drastic re-assemblies and compensatory regulations of voltage-gated (I\nKv\n) and Ca\n2+\n-activated (I\nK(Ca)\n) K\n+ \ncurrents during postembryonic development. Larval and adult neurons displayed prominent fast-inactivating I\nKv\n, mediated by the Shaker (Sh) channel to a large extent, while in the same neurons I\nK(Ca)\nwas far smaller in amplitude. In contrast, pupal neurons were characterized by large sustained I\nKv\nand prominent I\nK(Ca)\n, encoded predominantly by the slowpoke (slo) gene. Surprisingly, deletion of Sh in the Sh\nM\nnull mutant removed inactivating, transient I\nKv\nfrom large portions of neurons at all stages. Interestingly, elimination of Sh currents was accompanied by upregulation of non-Sh transient I\nKv\n. In comparison, the slo\n1\nmutation abolished the vast majority of I\nK(Ca)\n, particularly at the pupal stage. Strikingly, the deficiency of I\nK(Ca)\nin slo pupae was compensated by the transient component of I\nKv\nmediated by Sh channels. Thus, I\nK(Ca)\nappears to play critical roles in pupal development and its absence induces functional compensations from a specific transient I\nKv\ncurrent. While mutants lacking either Sh or slo currents survived normally, Sh;;slo double mutants deficient in both failed to survive through pupal metamorphosis. Together, our data highlight significant reorganizations and homeostatic compensations of K\n+ \ncurrents during postembryonic development and uncover previously unrecognized roles for Sh and slo in this plastic process.
Details
- Title: Subtitle
- K+ channel reorganization and homeostatic plasticity during postembryonic development: biophysical and genetic analyses in acutely dissociated Drosophila central neurons
- Creators
- Taixiang Saur - New England Primate Research Center, Harvard Medical SchoolI-Feng Peng - Department of Biology, University of IowaPeng Jiang - Institute of Neuroscience and Key Laboratory of Neurobiology, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesNeng Gong - Institute of Neuroscience and Key Laboratory of Neurobiology, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesWei-Dong Yao - New England Primate Research Center, Harvard Medical SchoolTian-Le Xu - Institute of Neuroscience and Key Laboratory of Neurobiology, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesChun-Fang Wu - Department of Biology, University of Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of neurogenetics, Vol.30(3-4), pp.259-275
- DOI
- 10.1080/01677063.2016.1255212
- PMID
- 27868467
- PMCID
- PMC5918286
- NLM abbreviation
- J Neurogenet
- ISSN
- 0167-7063
- eISSN
- 1563-5260
- Publisher
- Taylor & Francis
- Language
- English
- Date published
- 10/01/2016
- Academic Unit
- Iowa Neuroscience Institute; Biology; Computer Science
- Record Identifier
- 9984070245102771
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