Journal article
K+-evoked taurine efflux from cerebellar astrocytes: On the roles of Ca2+ and Na+
Neurochemical research, Vol.14(1), pp.43-48
01/1989
DOI: 10.1007/BF00969756
PMID: 2469025
Abstract
The ionic requirements for K+-evoked efflux of endogenous taurine from primary cerebellar astrocyte cultures were studied. The Ca2+ ionophore A23187 evoked taurine efflux in a dose-dependent fashion with a time-course identical to that of K+-induced efflux. The Ca2+-channel antagonist nifedipine had no effect upon efflux induced by 10 or 50 mM K+. In addition, verapamil did not antagonize 50 mM K+-evoked efflux except at high, non-pharmacological concentrations (greater than 100 microM), and preincubation with 2 microM omega-conotoxin had no effect on 50 mM K+-evoked efflux. Similarly, preincubation with 1 mM ouabain had no effect on the amount of taurine released by K+ stimulation, but did accelerate the onset of efflux by 2-4 min. Although 2 microM tetrodotoxin had no effect on K+-evoked release, replacing Na+ with choline abolished the taurine efflux seen in response to K+ stimulation. Together, these findings suggest that neuronal N- and L-type Ca2+- and voltage-dependent Na+-channels are not involved in the influx of Ca2+ which appears to be necessary for K+-evoked taurine efflux, and that in addition to Ca2+, extracellular Na+ is also required.
Details
- Title: Subtitle
- K+-evoked taurine efflux from cerebellar astrocytes: On the roles of Ca2+ and Na+
- Creators
- Robert A PhilibertKeith L RogersGary R Dutton
- Resource Type
- Journal article
- Publication Details
- Neurochemical research, Vol.14(1), pp.43-48
- DOI
- 10.1007/BF00969756
- PMID
- 2469025
- NLM abbreviation
- Neurochem Res
- ISSN
- 0364-3190
- eISSN
- 1573-6903
- Language
- English
- Date published
- 01/1989
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Psychiatry; Iowa Neuroscience Institute; Neuroscience and Pharmacology
- Record Identifier
- 9984004083002771
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