Kinetics of sevoflurane action on GABA- and glycine-induced currents in acutely dissociated rat hippocampal neurons

T Kira; N Harata; T Sakata; N Akaike

doi:10.1016/S0306-4522(97)00637-4

Back

Kinetics of sevoflurane action on GABA- and glycine-induced currents in acutely dissociated rat hippocampal neurons

Journal article

Peer reviewed

Kinetics of sevoflurane action on GABA- and glycine-induced currents in acutely dissociated rat hippocampal neurons

T Kira, N Harata, T Sakata and N Akaike

Neuroscience, Vol.85(2), pp.383-394

1998

DOI: 10.1016/S0306-4522(97)00637-4

PMID: 9622238

View Online

Abstract

Effects of a new kind of volatile anaesthetics, sevoflurane, on GABA- and glycine-gated chloride current (I Cl) were examined in single pyramidal neurons acutely dissociated from the rat hippocampal CA1 region, using the voltage-clamp mode of the nystatin-perforated patch-clamp technique. Rapid application of sevoflurane-induced I Cl by itself, with the time to peak reduced as the sevoflurane concentration was increased from 10 −3 to 3×10 −3 M. Although a pretreatment with 10 −3 M sevoflurane enhanced the peak amplitude of GABA (3×10 −6 M)-induced I Cl and suppressed the peak amplitude when the GABA concentration was increased to 10 −4 M, the pretreatment decreased the time to peak of the I Cl induced by any concentration of GABA (from 3×10 −6 to 10 −4 M). The treatment also accelerated the decay phase of the GABA-induced I Cl. On the other hand, sevoflurane suppressed the peak I Cl induced by 3×10 −5 M glycine in a concentration-dependent manner. In the presence of 3×10 −4 M sevoflurane, the peak amplitude of the glycine-induced I Cl was decreased without changes in EC 50 or Hill coefficients. Pretreatment with 10 −3 M sevoflurane did not affect the time to peak of the I Cl induced by any concentration of glycine (from 3×10 −5 to 10 −3 M). Pretreatment with 3×10 −8 M strychnine markedly prolonged the time to peak of the glycine-induced I Cl. These results suggest that sevoflurane modulated the amplitude of the GABA responses, depending on the balance of the accelerated activation and decay phases, and that sevoflurane suppressed the glycine-induced I Cl in a non-competitive manner without noticeable effect on the kinetics. The reversible and differential modulation of GABA A and glycine receptors might underlie a part of the anaesthetic actions and less adverse clinical effects of sevoflurane.

GABA

nystatin-perforated patch

sevoflurane

glycine

chloride current

hippocampal neuron

Details

Title: Subtitle: Kinetics of sevoflurane action on GABA- and glycine-induced currents in acutely dissociated rat hippocampal neurons
Creators: T Kira - Department of Internal Medicine I, School of Medicine, Oita Medical University, Oita 879-55, Japan
N Harata - Department of Physiology, Kyushu University Faculty of Medicine, Fukuoka 812-82, Japan
T Sakata - Department of Internal Medicine I, School of Medicine, Oita Medical University, Oita 879-55, Japan
N Akaike - Department of Physiology, Kyushu University Faculty of Medicine, Fukuoka 812-82, Japan
Resource Type: Journal article
Publication Details: Neuroscience, Vol.85(2), pp.383-394
Publisher: Elsevier Ltd
DOI: 10.1016/S0306-4522(97)00637-4
PMID: 9622238
ISSN: 0306-4522
eISSN: 1873-7544
Grant note: DOI: 10.13039/501100001700, name: Ministry of Education, Culture, Sports, Science and Technology
Language: English
Date published: 1998
Academic Unit: Molecular Physiology and Biophysics
Record Identifier: 9984025599202771

Metrics

11 Record Views

27 Times Cited - Web of Science